深圳大学学报(理工版)
深圳大學學報(理工版)
심수대학학보(리공판)
JOURNAL OF SHENZHEN UNIVERISYT SCIENCE & ENGINEERING
2010年
2期
236-240
,共5页
细胞生物学%细胞死亡%稀土元素%柠檬酸镧%失巢凋亡%肝癌细胞%HepG2细胞%线粒体膜电位
細胞生物學%細胞死亡%稀土元素%檸檬痠鑭%失巢凋亡%肝癌細胞%HepG2細胞%線粒體膜電位
세포생물학%세포사망%희토원소%저몽산란%실소조망%간암세포%HepG2세포%선립체막전위
cell biology%cell death%rare earth element%lanthanum citrate%anoikis%liver cancer cell%HepG2 cell%mitochondrial membrane potential
研究柠檬酸镧对肝癌细胞HepG2失巢凋亡的影响.采用聚羟乙基异丁烯酸阻断细胞与胞外基质联系的方法,建立体外细胞失巢生长模型.在贴壁和失巢条件下,用浓度0.1、0.01和0.001 mmol/L的柠檬酸镧溶液处理细胞.对于贴壁细胞,用碘化丙啶(propidium iodide,PI)染色检测细胞周期和凋亡率.对于失巢细胞,运用AnnexinV FITC-PI双染法检测失巢凋亡,用线粒体膜电位检测试剂盒JC-1(5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide)荧光显微镜法检测线粒体膜电位.结果表明,在贴壁条件下,柠檬酸镧处理组的周期和凋亡率与对照组相比,不存在明显差异.而在失巢条件下,柠檬酸镧促进HepG2细胞失巢凋亡,降低了线粒体膜电位.结果显示,稀土元素可能具有作为新的抗癌药物的潜力.
研究檸檬痠鑭對肝癌細胞HepG2失巢凋亡的影響.採用聚羥乙基異丁烯痠阻斷細胞與胞外基質聯繫的方法,建立體外細胞失巢生長模型.在貼壁和失巢條件下,用濃度0.1、0.01和0.001 mmol/L的檸檬痠鑭溶液處理細胞.對于貼壁細胞,用碘化丙啶(propidium iodide,PI)染色檢測細胞週期和凋亡率.對于失巢細胞,運用AnnexinV FITC-PI雙染法檢測失巢凋亡,用線粒體膜電位檢測試劑盒JC-1(5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide)熒光顯微鏡法檢測線粒體膜電位.結果錶明,在貼壁條件下,檸檬痠鑭處理組的週期和凋亡率與對照組相比,不存在明顯差異.而在失巢條件下,檸檬痠鑭促進HepG2細胞失巢凋亡,降低瞭線粒體膜電位.結果顯示,稀土元素可能具有作為新的抗癌藥物的潛力.
연구저몽산란대간암세포HepG2실소조망적영향.채용취간을기이정희산조단세포여포외기질련계적방법,건입체외세포실소생장모형.재첩벽화실소조건하,용농도0.1、0.01화0.001 mmol/L적저몽산란용액처리세포.대우첩벽세포,용전화병정(propidium iodide,PI)염색검측세포주기화조망솔.대우실소세포,운용AnnexinV FITC-PI쌍염법검측실소조망,용선립체막전위검측시제합JC-1(5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide)형광현미경법검측선립체막전위.결과표명,재첩벽조건하,저몽산란처리조적주기화조망솔여대조조상비,불존재명현차이.이재실소조건하,저몽산란촉진HepG2세포실소조망,강저료선립체막전위.결과현시,희토원소가능구유작위신적항암약물적잠력.
Effect of lanthanum citrate on the anoikis of human hepatoma (HepG2) cells was investigated in this study. Polyhydroxyethylmethacrylate (Poly-HEMA)was used to establish the non-attachment culture model by cell-contact blocking. HepG2 cells were treated with 0.1, 0.01 and 0.001 mmol/L lanthanum citrate under attachment condition and non-attachment condition. Cell cycles and apoptosis of HepG2 under attachment condition were detected by PI (propidium iodide) staining.Anoikis and mitochondrial membrane potential of HepG2 under non-attachment condition was measured by Annexin V-PI double staining and JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide) fluorescence microscope method respectively. Results showed that lanthanum citrate treatment brought no difference in cell cycle and anoikis to HepG2 under attachment condition but increased the anoikis and decreased mitochondrial membrane potential of HepG2 under non-attachment condition. These results implied that rare earth elements may have the potential as a new cancer treatment medicine.
