生物技术
生物技術
생물기술
BIOTECHNOLOGY
2010年
2期
56-58
,共3页
王红宁%左国伟%陈地龙%官涛%李春莉%姜蓉%李静%雷翠蓉%顾恒伟%王建伟
王紅寧%左國偉%陳地龍%官濤%李春莉%薑蓉%李靜%雷翠蓉%顧恆偉%王建偉
왕홍저%좌국위%진지룡%관도%리춘리%강용%리정%뢰취용%고항위%왕건위
人参皂苷单体Rb1 Rg1 Re%KG1α细胞%G_2/M期阻滞%增殖抑制
人參皂苷單體Rb1 Rg1 Re%KG1α細胞%G_2/M期阻滯%增殖抑製
인삼조감단체Rb1 Rg1 Re%KG1α세포%G_2/M기조체%증식억제
ginsenoside Rb1%KG1α cell%G_2/M phase arrest%inhibition of proliferation
目的:探讨人参皂苷Rb1、Rg1、Re对急性髓系白血病细胞株(KG1α)增殖的影响.方法:取对数生长期KG1α细胞,分设人参皂苷Rb1、Rg1、Re组和常规培养组,以MTT比色法检测作用24h、48h、72h时对KG1α细胞增殖抑制作用,并计算Rb1的IC_(50)值,以此浓度为工作浓度,设常规培养组和处理组,台盼蓝计数法观察对KG1α细胞增殖的影响;流式细胞术测定细胞周期分布的变化.结果:MTT、台盼蓝计数显示人参皂苷单体Rb1、Rg1可抑制KG1α细胞增殖,呈浓度依赖性,以Rb1抑制效应最佳,于作用48h抑制率最高.台盼蓝计数显示人参皂苷单体Rb1-120μmol/L作用48h时抑制率达50.22%;流式细胞术结果提示,与对照组比较,Rb1-120μmol/L组G_2/M期KG1α细胞比例增加(P<0.05).结论:Rb1可抑制KG1α细胞体外增殖,其增殖抑制作用与将KG1α细胞阻滞于G_2/M期有关.
目的:探討人參皂苷Rb1、Rg1、Re對急性髓繫白血病細胞株(KG1α)增殖的影響.方法:取對數生長期KG1α細胞,分設人參皂苷Rb1、Rg1、Re組和常規培養組,以MTT比色法檢測作用24h、48h、72h時對KG1α細胞增殖抑製作用,併計算Rb1的IC_(50)值,以此濃度為工作濃度,設常規培養組和處理組,檯盼藍計數法觀察對KG1α細胞增殖的影響;流式細胞術測定細胞週期分佈的變化.結果:MTT、檯盼藍計數顯示人參皂苷單體Rb1、Rg1可抑製KG1α細胞增殖,呈濃度依賴性,以Rb1抑製效應最佳,于作用48h抑製率最高.檯盼藍計數顯示人參皂苷單體Rb1-120μmol/L作用48h時抑製率達50.22%;流式細胞術結果提示,與對照組比較,Rb1-120μmol/L組G_2/M期KG1α細胞比例增加(P<0.05).結論:Rb1可抑製KG1α細胞體外增殖,其增殖抑製作用與將KG1α細胞阻滯于G_2/M期有關.
목적:탐토인삼조감Rb1、Rg1、Re대급성수계백혈병세포주(KG1α)증식적영향.방법:취대수생장기KG1α세포,분설인삼조감Rb1、Rg1、Re조화상규배양조,이MTT비색법검측작용24h、48h、72h시대KG1α세포증식억제작용,병계산Rb1적IC_(50)치,이차농도위공작농도,설상규배양조화처리조,태반람계수법관찰대KG1α세포증식적영향;류식세포술측정세포주기분포적변화.결과:MTT、태반람계수현시인삼조감단체Rb1、Rg1가억제KG1α세포증식,정농도의뢰성,이Rb1억제효응최가,우작용48h억제솔최고.태반람계수현시인삼조감단체Rb1-120μmol/L작용48h시억제솔체50.22%;류식세포술결과제시,여대조조비교,Rb1-120μmol/L조G_2/M기KG1α세포비례증가(P<0.05).결론:Rb1가억제KG1α세포체외증식,기증식억제작용여장KG1α세포조체우G_2/M기유관.
Objective:To investigate the effect of ginsenoside Rb1, Rg1 and Re on proliferation of KGI a cells.Method:Mter KG1 α cells were incubated with 20μmoL/L,40μmol/L,60μmol/L,80μmol/L, 100μmol/L, 120μmol/L, 160μmol/L, Rbl or 5μmol/L, 10μmol/L, 20μmul/L,40μmol/L ginsenoside Rg1 , 30μmol/L,60μmol/L,90μmol/L, 120μmol/L, 150μmol/L ginsenoside Re respectively for 24h, 48h and 72h, the conventional culture was performed in blank control group, the the effects of ginsenoside Rb1, Rg1, Re on the prolifera-tion of KG1α cells were examined by MTT assay.IC_(50) of ginsenoside Rb1 (120μmol/L) against KG1α cell for 48 hours was taken as the working concentration in following experiment, trypan blue staining was used to evaluate the ability to inhibit KG1α cell proliferation of ginsenoside Rb1, the effect of the ginsenoside Rb1 on KG1α cell cycle was determined by flow cytometry (FCM).Result: Ginsenoside Rb1 inhibited KG1α cells proliferation significantly in vitro, compared with Ginsenoside Rg1, Re, which exhibited a dose - dependent manner at range of 20 - 160 μmol/L , and reached peak at 48h.As determined by FCM analysis, ginseneside Rb1 arrested the cells in G_2/Mphase (P <0.05) ; in addition, no "subdiploid" peak appeared.Conclusion: The results suggested that ginsenoside Rb1 may in-lfibit the proliferation of KG1α cells ex vivo by arresting the cells in G_2/M phase.
