CAJ | 학술논문

目的探讨2000 μW/cm2电磁辐射对大鼠海马N-甲基-D-门冬氨酸(NMDA)受体NR1亚单位蛋白及其mRNA水平表达的影响,揭示电磁辐射对大鼠学习记忆功能的损伤机制.方法实验分为空白对照组,假辐射组,1 h/d、2h/d、3 h/d辐射组.将辐射组大鼠固定体位,头部接受功率密度为2000μW/cm2的近场辐射,连续辐射30d.通过Morris水迷宫检测大鼠的空间学习记忆能力,采用免疫组化法和Western-Blot法检测大鼠海马组织NR1蛋白表达的变化,RT-PCR法检测大鼠海马组织NR1 mRNA表达的变化.结果各辐射组大鼠在水迷宫检测第4天寻找安全平台的逃避潜伏期分别为1 h/d[(12.29±1.36)s]、2 h/d[(17.99±2.25)s]、3 h/d[(24.66±5.56)s],均明显长于空白对照组[(8.8±1.66)s](P＜0.05);1 h/d、2 h/d和3 h/d辐射组大鼠海马神经元均排列紊乱,NR1阳性细胞比率明显低于空白对照组,海马组织NR1蛋白[分别为(0.122±0.026)、(0.102±0.023)、(0.060±0.009)]及其mRNA[分别为(0.46±0.07)、(0.35±0.05)、(0.12±0.02)]表达水平较空白对照组[(10.70±0.11)、(0.68±0.11)]均明显降低(P＜0.05).而假辐射组大鼠各项指标与空白对照组相比均无显著差异(P＞0.05).结论 2000μW/cm2电磁辐射可导致大鼠学习记忆功能下降,其机制可能与大鼠海马组织NR1蛋白及其mRNA的表达降低有关.
목적 탐토2000 μW/cm2전자복사대대서해마N-갑기-D-문동안산(NMDA)수체NR1아단위단백급기mRNA수평표체적영향,게시전자복사대대서학습기억공능적손상궤제.방법 실험분위공백대조조,가복사조,1 h/d、2h/d、3 h/d복사조.장복사조대서고정체위,두부접수공솔밀도위2000μW/cm2적근장복사,련속복사30d.통과Morris수미궁검측대서적공간학습기억능력,채용면역조화법화Western-Blot법검측대서해마조직NR1단백표체적변화,RT-PCR법검측대서해마조직NR1 mRNA표체적변화.결과 각복사조대서재수미궁검측제4천심조안전평태적도피잠복기분별위1 h/d[(12.29±1.36)s]、2 h/d[(17.99±2.25)s]、3 h/d[(24.66±5.56)s],균명현장우공백대조조[(8.8±1.66)s](P＜0.05);1 h/d、2 h/d화3 h/d복사조대서해마신경원균배렬문란,NR1양성세포비솔명현저우공백대조조,해마조직NR1단백[분별위(0.122±0.026)、(0.102±0.023)、(0.060±0.009)]급기mRNA[분별위(0.46±0.07)、(0.35±0.05)、(0.12±0.02)]표체수평교공백대조조[(10.70±0.11)、(0.68±0.11)]균명현강저(P＜0.05).이가복사조대서각항지표여공백대조조상비균무현저차이(P＞0.05).결론 2000μW/cm2전자복사가도치대서학습기억공능하강,기궤제가능여대서해마조직NR1단백급기mRNA적표체강저유관.
Objective To evaluate the effects of electromagnetic irradiation of 2000 μW/cm2 exposure on mRNA and protein expression levels of immunoreactive protein and mRNA of NMDAR1 in rats hippocampal,and to explore the impaired mechanism of electromagnetic irradiation on learning and memory.Methods Rats were randomly divided into normal control group, sham-radiated group, and 1 h/d, 2 h/d, and 3 h/d radiation groups.The rats in the radiation groups were fixed and recieved microwave exposure of 2000 μW/cm2, then their learning and memory abilities were tested by Morris water maze experiment, the change of NR1 protein in hippocampal neurons of each group of rats was measured with immunohistochmistry and western blot techniques, and the expression of NR1 mRNA in hippocampus was determined by RT-PCR.Results In the water maze test,compared with the normal control group (8.8 ± 1.66 ), the escape latency of three radiated groups rats ( 1 h/d ( 12.29 ±1.36) s,2 h/d ( 17.99 ±2.25) s,and 3 h/d (24.66 ±5.56) s) were significantly longer (P＜0.05).In the radiation group,the hippocampal neurons of rats showed evident reduction in the ratio of NR1 positive cells,irregular,and arrayed in disorder.Moreover,compared with the normal control group ( (0.70 ±0.11 ), (0.68 ±0.11 ) ) ,the expession of NR1 protein ( 1 h/d (0.122 ±0.026) ,2 h/d (0.102 ±0.023) ,and 3 h/d (0.060 ± 0.009) ) and its mRNA ( 1 h/d (0.46 ±0.07) ,2 h/d (0.35 ±0.05) ,and 3 h/d (0.12 ±0.02) ) in hippocampal neurons was significantly decreased (P＜0.05).Among the indicators, there was no significant difference between sham-radiated group and normal control group.Conclusions Electromagnetic irradiation of 2000 μW/cm2 exposure can impair the learning and memory abilities of rats possibly through a mechanism correlated with the lower expression of NR1 protein and its mRNA in hippocampus.