中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2012年
3期
274-277
,共4页
淋巴瘤%乏氧诱导因子-1α%生存素%电离辐射
淋巴瘤%乏氧誘導因子-1α%生存素%電離輻射
림파류%핍양유도인자-1α%생존소%전리복사
Lymphoma%Hypoxia inducible factor-1α%Survivin%Ionizing radiation
目的 研究电离辐射对人非霍奇金淋巴瘤细胞中HIF-1α-Survivin通路活化状态的影响,探讨恶性淋巴瘤放射抗性的机制.方法 采用Western blot方法检测辐射后3种淋巴瘤细胞中HIF-1α和Survivin蛋白的表达水平,观察应用HIF-1α抑制剂Echinomycin及转染反义HIF-1a siRNA对Survivin蛋白及mRNA表达的影响.结果 在人非霍奇金淋巴瘤细胞中存在HIF-1α和Survivin蛋白的表达,X射线照射后10～20 h出现HIF～1α蛋白表达增加,照射后24 h Survivin蛋白表达增加,与对照组比较差异有统计学意义(t =7.53 ～31.31,P＜0.01).与单纯照射组比较,采用HIF-1α抑制剂预处理肿瘤细胞后,Survivin蛋白表达下降,且具有药物浓度依赖性(t=7.21 ～32.81,P＜0.01).而转染反义HIF-1α siRNA后,照射未诱导产生survivin mRNA和蛋白表达增加.结论 电离辐射能够活化人非霍奇金淋巴瘤细胞中的HIF-1α-Survivin通路,可能与肿瘤的放射抗性有关.
目的 研究電離輻射對人非霍奇金淋巴瘤細胞中HIF-1α-Survivin通路活化狀態的影響,探討噁性淋巴瘤放射抗性的機製.方法 採用Western blot方法檢測輻射後3種淋巴瘤細胞中HIF-1α和Survivin蛋白的錶達水平,觀察應用HIF-1α抑製劑Echinomycin及轉染反義HIF-1a siRNA對Survivin蛋白及mRNA錶達的影響.結果 在人非霍奇金淋巴瘤細胞中存在HIF-1α和Survivin蛋白的錶達,X射線照射後10～20 h齣現HIF～1α蛋白錶達增加,照射後24 h Survivin蛋白錶達增加,與對照組比較差異有統計學意義(t =7.53 ～31.31,P＜0.01).與單純照射組比較,採用HIF-1α抑製劑預處理腫瘤細胞後,Survivin蛋白錶達下降,且具有藥物濃度依賴性(t=7.21 ～32.81,P＜0.01).而轉染反義HIF-1α siRNA後,照射未誘導產生survivin mRNA和蛋白錶達增加.結論 電離輻射能夠活化人非霍奇金淋巴瘤細胞中的HIF-1α-Survivin通路,可能與腫瘤的放射抗性有關.
목적 연구전리복사대인비곽기금림파류세포중HIF-1α-Survivin통로활화상태적영향,탐토악성림파류방사항성적궤제.방법 채용Western blot방법검측복사후3충림파류세포중HIF-1α화Survivin단백적표체수평,관찰응용HIF-1α억제제Echinomycin급전염반의HIF-1a siRNA대Survivin단백급mRNA표체적영향.결과 재인비곽기금림파류세포중존재HIF-1α화Survivin단백적표체,X사선조사후10～20 h출현HIF～1α단백표체증가,조사후24 h Survivin단백표체증가,여대조조비교차이유통계학의의(t =7.53 ～31.31,P＜0.01).여단순조사조비교,채용HIF-1α억제제예처리종류세포후,Survivin단백표체하강,차구유약물농도의뢰성(t=7.21 ～32.81,P＜0.01).이전염반의HIF-1α siRNA후,조사미유도산생survivin mRNA화단백표체증가.결론 전리복사능구활화인비곽기금림파류세포중적HIF-1α-Survivin통로,가능여종류적방사항성유관.
Objective To determine the levels of radiation-induced expressions of HIF-1α and Survivin in three human lymphoma cell lines with or without HIF-1a specific antagonist or HIF-1a siRNA transfection and to explore the possible mechanism of cell radioresistance.Methods Namalwa,Ramos,Raji cells were irradiated with X-rays with or without the combination of echinomycin (EC),a HIF-1α specific inhibitor,or transfection of HIF-1α siRNA.Then the expression levels of HIF-1α and Survivin proteins and mRNAs were determined by Western blot and real-time PCR,respectively.Results After irradiation,the expression level of HIF-1α protein was up-regulated at 10-20 h followed by up-regulation of survivin protein at 24 h (t =7.53-31.31,P ＜ 0.01 ) in three lymphoma cell lines.Radiation-induced up-expression of survivin protein was significantly reduced by either HIF-1α inhibition or EC treatment (t =7.21-32.81,P ＜0.01 ).However,radiation failed to induce up-erpression of survivin at both mRNA and protein levels after transfection with HIF-1α siRNA.Conclusion HIF-1α-Survivin pathway is activated in human lymphoma cells after radiation,which might be involved in the cell radioresistance.
