中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2011年
11期
962-964
,共3页
祝鑫海%王永清%姜志明%吴稚冰
祝鑫海%王永清%薑誌明%吳稚冰
축흠해%왕영청%강지명%오치빙
癌,非小细胞肺%顺铂%细胞因子信号转导蛋白抑制因子
癌,非小細胞肺%順鉑%細胞因子信號轉導蛋白抑製因子
암,비소세포폐%순박%세포인자신호전도단백억제인자
Carcinoma,non-small-cell lung%Cisplatin%Suppressor of cytokine signaling proteins
目的 探讨β-榄香烯联合顺铂和热疗对非小细胞肺癌A549细胞系杀伤的可能生物学机制.方法 不同浓度榄香烯联合热疗和顺铂处理细胞后,用Western blot检测Star3、p21 Wafl/Cip1、Survivin蛋白表达.结果 37℃下随着榄香烯浓度的升高,A549细胞中Stat3、磷酸化Stat3(pStat3)和p21 Waf1/Cip1蛋白的表达均升高,且高、低浓度组间差异有统计学意义,Survivin蛋白未见明显变化；联合4 μg/ml顺铂后,高浓度榄香烯下p21Waf1/Cip1蛋白、Survivin蛋白以及Star3和pStat3蛋白的表达全部降低；42℃下,榄香烯60 μg/ml组的Stat3蛋白无明显变化,但是pStat3蛋白、p21 Waf1/Cip1蛋白和Survivin蛋白均显著下降；榄香烯15 μg/ml联合4 μg/ml顺铂组Stat3和pStat3蛋白表达均上升,而Survivin表达降低.结论 37℃下高浓度榄香烯通过p21Waf1/Cip1蛋白高表达抑制A549细胞的生长；高浓度榄香烯联合顺铂主要通过抑制Stat3和pStat3蛋白的表达及使Survivin蛋白降低,抑制细胞生长；42℃下,高浓度榄香烯可能通过抑制Stat3磷酸化为pStat3的过程降低Survivin蛋白表达,促进细胞凋亡；低浓度榄香烯联合顺铂通过降低Survivin蛋白表达导致协同杀伤效应.
目的 探討β-欖香烯聯閤順鉑和熱療對非小細胞肺癌A549細胞繫殺傷的可能生物學機製.方法 不同濃度欖香烯聯閤熱療和順鉑處理細胞後,用Western blot檢測Star3、p21 Wafl/Cip1、Survivin蛋白錶達.結果 37℃下隨著欖香烯濃度的升高,A549細胞中Stat3、燐痠化Stat3(pStat3)和p21 Waf1/Cip1蛋白的錶達均升高,且高、低濃度組間差異有統計學意義,Survivin蛋白未見明顯變化；聯閤4 μg/ml順鉑後,高濃度欖香烯下p21Waf1/Cip1蛋白、Survivin蛋白以及Star3和pStat3蛋白的錶達全部降低；42℃下,欖香烯60 μg/ml組的Stat3蛋白無明顯變化,但是pStat3蛋白、p21 Waf1/Cip1蛋白和Survivin蛋白均顯著下降；欖香烯15 μg/ml聯閤4 μg/ml順鉑組Stat3和pStat3蛋白錶達均上升,而Survivin錶達降低.結論 37℃下高濃度欖香烯通過p21Waf1/Cip1蛋白高錶達抑製A549細胞的生長；高濃度欖香烯聯閤順鉑主要通過抑製Stat3和pStat3蛋白的錶達及使Survivin蛋白降低,抑製細胞生長；42℃下,高濃度欖香烯可能通過抑製Stat3燐痠化為pStat3的過程降低Survivin蛋白錶達,促進細胞凋亡；低濃度欖香烯聯閤順鉑通過降低Survivin蛋白錶達導緻協同殺傷效應.
목적 탐토β-람향희연합순박화열료대비소세포폐암A549세포계살상적가능생물학궤제.방법 불동농도람향희연합열료화순박처리세포후,용Western blot검측Star3、p21 Wafl/Cip1、Survivin단백표체.결과 37℃하수착람향희농도적승고,A549세포중Stat3、린산화Stat3(pStat3)화p21 Waf1/Cip1단백적표체균승고,차고、저농도조간차이유통계학의의,Survivin단백미견명현변화；연합4 μg/ml순박후,고농도람향희하p21Waf1/Cip1단백、Survivin단백이급Star3화pStat3단백적표체전부강저；42℃하,람향희60 μg/ml조적Stat3단백무명현변화,단시pStat3단백、p21 Waf1/Cip1단백화Survivin단백균현저하강；람향희15 μg/ml연합4 μg/ml순박조Stat3화pStat3단백표체균상승,이Survivin표체강저.결론 37℃하고농도람향희통과p21Waf1/Cip1단백고표체억제A549세포적생장；고농도람향희연합순박주요통과억제Stat3화pStat3단백적표체급사Survivin단백강저,억제세포생장；42℃하,고농도람향희가능통과억제Stat3린산화위pStat3적과정강저Survivin단백표체,촉진세포조망；저농도람향희연합순박통과강저Survivin단백표체도치협동살상효응.
Objective To explore the possible molecular mechanisms of β-elemene combined with cisplatin and heat therapy for killing A549 cell line.Methods The protein expressions of Stat3,p21 Waf1/Cip1 and Survivin were detected with Western blot after treatment with β-elemene of different concentrations combined cisplatin and heat therapy.Results The protein expressions of Stat3,and pStat3 and p21Waf1/Cip1 in A549 cell line were enhanced with increasing concentrations,and there was significant difference in the expressions between high and low concentration,but Survivin protein had no change at 37°C.After adding 4 μg/mL cisplatin,the expressions of p21Waf1/ Cip1,Survivin,Stats and pStat3 were reduced at β-elemene of high concentration.At 42°C,there was no significant difference in expression of Stat3 protein at 60 μg/mL elemene,but the expressions of pStat3,p21Waf1/Cip1 and Survivin proteins had sharply declined.When using 15 μg/mL elemene combined with 4 μg/mL cisplatin,the protein expressions of Star3 and pStat3 increased,and Survivin expression decreased.Conclusions At temperature of 37°C,β-elemene of high concentration may inhibit growth of A549 cells by higher expression of p21Waf1/Cip1 protein,and mainly by inhibiting expressions of Stat3 and pStat3 and Survivin after combined with cisplatin.At temperature of 42°C,β- elemene of high concentration may promote apoptosis possibly through inhibition of Stat3 phosphorylation and expression of Survivin protein.β-elemene of low concentration combined with cisplatin leads to synergy killing effect by reducing expression of Survivin protein.
