CAJ | 학술논문

目的探讨环氧合酶-2(COX-2)、第10号染色体同源缺失性磷酸酶-张力蛋白基因(phosphatase and tensin homolog deleted on chromosome ten,PTEN)在肝胆管结石合并胆管癌(hepatobiliary calculus with cholangiocarcinoma,HCWC)中的表达及其意义,研究COX-2、PTEN与HCWC发生、发展的关系,为HCWC的临床防治提供依据.方法研究分三组:正常胆管组(肝血管瘤或肝外伤切除标本)10例(A组),肝内结石所在胆管组30例(B组),HCWC组37例(C组).应用免疫组织化学SP法,通用二步法检测各组COX-2、PTEN的表达情况,并对检测结果进行分析.结果 COX-2在A、B、C三组中的表达阳性率分别为10.0%、33.3%和70.3%,癌组织中的表达阳性率明显高于对照组胆管组织(P＜0.01).PTEN在A、B和C三组中的表达阳性率分别为90.0%、80.0%和35.1%,癌组织中的表达阳性率明显低于对照组胆管组织(P＜0.01).在COX-2阳性表达的HCWC组织中,PTEN有较低表达.Kendall相关分析表明HCWC组织中COX-2与PTEN表达呈显著负相关(r=-0.323,P＜0.05).结论 COX-2高表达可能与HCWC形成有关.在HCWC中,COX-2和PTEN表达呈负相关.COX-2高表达、PTEN低表达可能提示HCWC更易发生淋巴转移或肝外转移.
목적 탐토배양합매-2(COX-2)、제10호염색체동원결실성린산매-장력단백기인(phosphatase and tensin homolog deleted on chromosome ten,PTEN)재간담관결석합병담관암(hepatobiliary calculus with cholangiocarcinoma,HCWC)중적표체급기의의,연구COX-2、PTEN여HCWC발생、발전적관계,위HCWC적림상방치제공의거.방법 연구분삼조:정상담관조(간혈관류혹간외상절제표본)10례(A조),간내결석소재담관조30례(B조),HCWC조37례(C조).응용면역조직화학SP법,통용이보법검측각조COX-2、PTEN적표체정황,병대검측결과진행분석.결과 COX-2재A、B、C삼조중적표체양성솔분별위10.0%、33.3%화70.3%,암조직중적표체양성솔명현고우대조조담관조직(P＜0.01).PTEN재A、B화C삼조중적표체양성솔분별위90.0%、80.0%화35.1%,암조직중적표체양성솔명현저우대조조담관조직(P＜0.01).재COX-2양성표체적HCWC조직중,PTEN유교저표체.Kendall상관분석표명HCWC조직중COX-2여PTEN표체정현저부상관(r=-0.323,P＜0.05).결론 COX-2고표체가능여HCWC형성유관.재HCWC중,COX-2화PTEN표체정부상관.COX-2고표체、PTEN저표체가능제시HCWC경역발생림파전이혹간외전이.
Objective To explore the expressions of cyclooxygenase-2 (COX-2), phosphatase and tensin homolog deleted on chromosome ten (PTEN) in hepatobiliary calculus associated with cholangiocarcinoma (HCWC) and their clinical significance. The relationship between the expressions of COX-2, PTEN and the onset and progression of HCWC was investigated to form an experimental base for the prevention and treatment of HCWC. Methods Thirty seven patients with tumor tissues of HCWC (group C), thirty patients with tissues of bile duct surrounding intrahepatic calculus (group B), and ten patients with normal tissues of bile duct from operations of hemangiomas of liver or liver trauma as the control (group A) were sampled and collected. A two-step immunohistochemistry (SP method) was employed to detect and statistically analyze the expressions of COX-2 and PTEN in each of the 3 groups. Results In groups A, B, C, the positive rate of the expression of COX-2 was 10%,33.3%, and 70.3%, respectively. The positive rates of expression of COX-2 in the carcinoma tissues of HCWC was significantly higher compared with the control group (P＜0. 01). In groups A, B, C the positive rates of the expression of PTEN was 90. 0%, 80. 0%, and 35.0%, respectively. The positive rate of expression of PTEN in the carcinoma tissues of HCWC was significantly lower than the control group (P＜0. 01). The expression of COX-2 was followed by a low expression of PTEN in the tissues of HCWC. Kendall's related analysis showed a strong negative correlation between the expression of COX-2 and PTEN in HCWC (r=-0. 323, P＜0. 05). Conclusions A high expression of COX-2 was related to HCWC. There was a negative correlation between the expressions of COX-2 and PTEN in HCWC. A high expression of COX-2 and a low expression of PTEN suggested a high chance of HCWC in extrahepatic or lymphatic metastasis.