CAJ | 학술논문

目的观察电针联合康复训练对脊髓损伤(SCI)大鼠脑源性神经营养因子(BDNF)及其受体酪氨酸激酶B(TrkB)表达的影响.方法选取96只成年SD雌性大鼠,采用脊髓切割损伤法制作右侧脊髓半横断损伤模型.将制模成功大鼠随机分为电针组、康复训练组、联合治疗组及模型组.于制模后第3天各组大鼠按既定方案给予相应干预,其中电针组、康复训练组分别给予电针督脉穴位或康复训练,联合治疗组于康复训练后辅以电针刺激.每周均对各组大鼠进行BBB运动功能评分；于制模后第4周及第8周时每组分别取12只大鼠处死,采用免疫组化法、PCR及RT-PCR技术检测各组大鼠受损脊髓BDNF及TrkB表达情况.结果各组大鼠BBB评分、BDNF及其受体TrkB表达均随时间延长逐渐增加,其中电针组、康复训练组及联合治疗组上述指标在制模后第4周、第8周时均明显优于模型组(P＜0.05),同时联合治疗组也显著优于电针组及康复训练组(P＜0.05)；电针组及康复训练组的上述指标组间差异均无统计学意义(P＞0.05).结论电针督脉穴位联合康复训练治疗SCI大鼠具有协同疗效,能进一步加速大鼠运动功能恢复,其治疗机制可能与上调受损脊髓BDNF及其受体TrkB表达有关.
목적 관찰전침연합강복훈련대척수손상(SCI)대서뇌원성신경영양인자(BDNF)급기수체락안산격매B(TrkB)표체적영향.방법 선취96지성년SD자성대서,채용척수절할손상법제작우측척수반횡단손상모형.장제모성공대서수궤분위전침조、강복훈련조、연합치료조급모형조.우제모후제3천각조대서안기정방안급여상응간예,기중전침조、강복훈련조분별급여전침독맥혈위혹강복훈련,연합치료조우강복훈련후보이전침자격.매주균대각조대서진행BBB운동공능평분；우제모후제4주급제8주시매조분별취12지대서처사,채용면역조화법、PCR급RT-PCR기술검측각조대서수손척수BDNF급TrkB표체정황.결과 각조대서BBB평분、BDNF급기수체TrkB표체균수시간연장축점증가,기중전침조、강복훈련조급연합치료조상술지표재제모후제4주、제8주시균명현우우모형조(P＜0.05),동시연합치료조야현저우우전침조급강복훈련조(P＜0.05)；전침조급강복훈련조적상술지표조간차이균무통계학의의(P＞0.05).결론 전침독맥혈위연합강복훈련치료SCI대서구유협동료효,능진일보가속대서운동공능회복,기치료궤제가능여상조수손척수BDNF급기수체TrkB표체유관.
Objective To observe the changes of expressions of brain-derived neurotrophic factor (BDNF)and its receptor tyrosine kinase B(TrkB)and in rats with hemitransectional spinal cord injury(SCI)after electroacupuncture on Du Meridian and rehabilitation training.Methods The animal model of acute hemitransectional lesion at the right half of T11 spinal cord was established in 96 adult female rats,which were then divided randomly into an electroacupuncture group,a rehabilitation training group,an electroacupuncture combined with rehabilitation training group and a control group.All the groups received treatment on the 3rd d after operation.The electroacupuncture group and rehabilitation training group were given electroacupuncture on points of Du Meridian and rehabilitation training,respectively,and the combined group was given Du Meridian electroacupuncture in addition to rehabilitation training.Basso-Beattie-Bresnahan(BBB)scale was used to evaluate motor function every week.Twelve rats of each group were sacrificed 4 and 8 weeks after operation,respectively,and their spinal cord tissues were extracted.The polymerase chain reaction(PCR),reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemical techniques were used to detect the expressions of BDNF and TrkB.Results BBB grade increased gradually as time went on.There were significant differences between control group and other groups at the same time point(P ＜ 0.05).The scores increased obviously in electroacupuncture combined with rehabilitation training group compared with electroacupuncture group and rehabilitation training group(P ＜ 0.05).The result of immunohistochemical observation and RT-PCR also showed that there were significant differences of expressions of BDNF and TrkB among control group and other groups at the same time(P ＜ 0.05).The effects in electroacupuncture combined with rehabilitation training group were much more obvious than those in electroacupuncture group and rehabilitation training group(P ＜ 0.05),but there was no significant difference between electroacupuncture group and rehabilitation training group (P ＞ 0.05).Conclusions Electroacupuncture on Du Meridian combined with rehabilitation training had synergic effect on rat's SCI,and could obviously improve the restoration of rat's motor function; the mechanism maybe related to the upregulation of expressions of BDNF and TrkB.