CAJ | 학술논문

目的探讨B7-H1和PD-1信号通路与胃癌发生的关系.方法利用免疫组化技术和ANAE染色技术检测胃组织中B7-H1和PD-1的原位表达及肿瘤浸润淋巴细胞(TIL)浸润情况,利用逆转录聚合酶链反应(RT-PCR)技术检测胃癌组织中B7-H1和PD-1 mRNA的表达,利用Western blot技术检测B7-H1蛋白的表达.结果 B7-H1和PD-1在胃癌组织中表达升高,阳性率分别为64.4%和43.8%,而在正常胃组织中不表达.胃癌组织中,B7-H1表达与TIL浸润负相关.B7-H1表达与胃癌的浸润深度、周围淋巴结转移、远处转移及pTNM分期有关,而PD-1与其无关.结论胃癌组织中B7-H1和PD-1表达升高,B7-H1/PD-1信号通路可能抑制抗肿瘤免疫.B7-H1的表达与胃癌的病程有关,有可能作为判断胃癌预后的指标和胃癌治疗的靶点.
목적 탐토B7-H1화PD-1신호통로여위암발생적관계.방법 이용면역조화기술화ANAE염색기술검측위조직중B7-H1화PD-1적원위표체급종류침윤림파세포(TIL)침윤정황,이용역전록취합매련반응(RT-PCR)기술검측위암조직중B7-H1화PD-1 mRNA적표체,이용Western blot기술검측B7-H1단백적표체.결과 B7-H1화PD-1재위암조직중표체승고,양성솔분별위64.4%화43.8%,이재정상위조직중불표체.위암조직중,B7-H1표체여TIL침윤부상관.B7-H1표체여위암적침윤심도、주위림파결전이、원처전이급pTNM분기유관,이PD-1여기무관.결론 위암조직중B7-H1화PD-1표체승고,B7-H1/PD-1신호통로가능억제항종류면역.B7-H1적표체여위암적병정유관,유가능작위판단위암예후적지표화위암치료적파점.
Objective The B7-H1/PD-1 co-signaling pathway has recently been found to play a pivotal role in the immune evasion of tumor cells from host immune system.The aim of this study was to examine the B7-H1 and PD-1 expression and TILs status in gastric cancer and to elucidate the clinical relevance of B7-H1 and PD-1 to the pathogenesis of gastric carcinoma. Methods Immunohistochemistry and ANAE histochemical staining were used to investigate the in situ expression of B7-H1 and PD-1 and TILs status in the gastric tissues.RT-PCR was used to explore B7-H1 and PD-1 expression at the transcriptional level.The B7-H1 expression at protein level was detected by Western blot.Results Expression of B7-H1 and PD-1 was found to be increased in gastric carcinoma,but absent in normal gastric tissue.B7-H1 expression in gastric carcinoma was inversely correlated with TILs infiltration.B7-H1 but not PD-1 expression in tumor tissue was significantly correlated with some clinicopathhological variables including depth of invasion,lymph node metastasis and distant metastasis.Conclusion B7-H1 and PD-1 expressions are increased in gastric carcinoma.This signaling pathway may inhibit antitumor immune responses in gastric carcinoma.B7-H1 expression plays a critical role in the pathogenesis of human gastric carcinoma,and might be a promising prognostic marker and therapeutic target in the treatment of this disease.