CAJ | 학술논문

铜诱导基因表达系统已被用于植物转基因表达的时空调控.它由两部分构成:(1)组成型或器官专一性表达的acel基因编码铜反应性的转录因子;(2)融合启动子控制下的目的基因,融合启动子由含ACE1结合位点的金属反应元件(MRE)连接CaMV 35S(-90～+8)启动子组成.本实验测试了来自酵母金属硫蛋白基因5′调控区的两个不同的ACE1结合区域在转基因烟草(Nicotiana tabacum L. cv.W38)中的效果.结果表明,与MRE(-148～-105)相比,使用MRE(-210～-126)的铜诱导系统效率增加50%到100%.在植物生物技术中使用这一系统控制基因性状是很有潜力的.
동유도기인표체계통이피용우식물전기인표체적시공조공.타유량부분구성:(1)조성형혹기관전일성표체적acel기인편마동반응성적전록인자;(2)융합계동자공제하적목적기인,융합계동자유함ACE1결합위점적금속반응원건(MRE)련접CaMV 35S(-90～+8)계동자조성.본실험측시료래자효모금속류단백기인5′조공구적량개불동적ACE1결합구역재전기인연초(Nicotiana tabacum L. cv.W38)중적효과.결과표명,여MRE(-148～-105)상비,사용MRE(-210～-126)적동유도계통효솔증가50%도100%.재식물생물기술중사용저일계통공제기인성상시흔유잠력적.
The copper-regulated gene expression system has been developed to control spacial and temporal expression of transgene in plant. It comprises two parts: (1) ace1 gene encoding copper-responsive transcription factor under the control of a constitutive or organ-specific promoter, and (2) a gene of interest under the control of a chimeric promoter consisting of the CaMV 35S (-90 to +8) promoter linked to the metal responsive element (MRE) carrying activating copper-metallothionein expression (ACE1)binding sites. Here, the effectiveness of two different ACE1-binding cis -elements which derive from 5'-regulatory region of yeast metallothionein gene was investigated in transgenic tobacco (Nicotiana tabacum L. cv. W38). The results revealed that the MRE (-210 to -126) could increase the system inducibility by 50% - 100% compared with the previously reported MRE (-148 to -105). It is potential to use the copperinducible system to control valuable gene traits in plant biotechnology.