稀土学报（英文版）
稀土學報（英文版）
희토학보（영문판）
JOURNAL OF RARE EARTHS
2004年
5期
691-695
,共5页
botany%pueraria lobata%hairy root culture%isoflavonoid%tissue viability%rare earths
The effects of various rare earth elements on growth and isoflavonoid production in hairy root cultures of Pueraria lobata(Willd.) Ohwi(strain TR2) cultured in 500 ml flasks were studied. After 32 days of culture,the biomass of hairy roots increase 15 times and reach 3.2 g dry weight. Hairy root growth was inhibited by Y2O3,NaSeO3 and Sm3+ because of brown formation. But significant promoting effect on root growth due to callus formation was observed in La3+ treatments. It is the most noteworthy that the production of total isoflavonoids and puerarin was enhanced greatly by La3+ treatment. A major portion of increased total isoflavonoids and puerarin was released into medium in La3+ treatment while the hairy root viabilities were preserved. Some specific secondary metabolite release processes could be induced by La3+ and their possible mechanism is discussed.
