时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2009年
12期
2963-2964
,共2页
窦玉红%崔力剑%黄芸%李清
竇玉紅%崔力劍%黃蕓%李清
두옥홍%최력검%황예%리청
贞灵固本片%固相萃取%高效液相色谱%黄芪甲苷
貞靈固本片%固相萃取%高效液相色譜%黃芪甲苷
정령고본편%고상췌취%고효액상색보%황기갑감
Zhenlingguben Tablets%Solid-phase extraction%HPLC%Astragaloside Ⅳ
目的 建立贞灵固本片中黄芪甲苷的含量测定方法.方法 采用固相萃取-高效液相色谱-蒸发光散射检测法(SPE-HPLC-ELSD),Agilent-C_18色谱柱(4.6 mm×250 mm, 5 μm),流动相甲醇-水(74∶26),柱温35℃ ,流速0.8 ml·min~(-1).结果 黄芪甲苷在0.272～5.432 μg范围内具有良好的线性关系,r=0.999 9;平均加样回收率为98.82%,RSD为1.57%(n＝9).结论 该方法简便、快速、灵敏、准确、重复性好,可作为贞灵固本片的质量控制方法.
目的 建立貞靈固本片中黃芪甲苷的含量測定方法.方法 採用固相萃取-高效液相色譜-蒸髮光散射檢測法(SPE-HPLC-ELSD),Agilent-C_18色譜柱(4.6 mm×250 mm, 5 μm),流動相甲醇-水(74∶26),柱溫35℃ ,流速0.8 ml·min~(-1).結果 黃芪甲苷在0.272～5.432 μg範圍內具有良好的線性關繫,r=0.999 9;平均加樣迴收率為98.82%,RSD為1.57%(n＝9).結論 該方法簡便、快速、靈敏、準確、重複性好,可作為貞靈固本片的質量控製方法.
목적 건립정령고본편중황기갑감적함량측정방법.방법 채용고상췌취-고효액상색보-증발광산사검측법(SPE-HPLC-ELSD),Agilent-C_18색보주(4.6 mm×250 mm, 5 μm),류동상갑순-수(74∶26),주온35℃ ,류속0.8 ml·min~(-1).결과 황기갑감재0.272～5.432 μg범위내구유량호적선성관계,r=0.999 9;평균가양회수솔위98.82%,RSD위1.57%(n＝9).결론 해방법간편、쾌속、령민、준학、중복성호,가작위정령고본편적질량공제방법.
Objective To build up a method for determination of astragaloside Ⅳ in Zhenlingguben tablets. Methods SPE-HPLC-ELSD was used in the quantitative analysis with Agilent C_18 chromatography column (4.6 mm×250 mm, 5μm) and methanol-water (74∶26) as a mobile phase. The flow rate of mobile phase was 0.8 ml·min~(-1). The column temperature was set to 35℃.Results The linear range for astragaloside Ⅳ was 0.272～5.432μg (r=0.999 9), the average recovery was 98.82% (RSD=1.57%, n=9).Conclusion This method is simple, rapid, reproducible, and can be regarded as the quality control method of Zhenlingguben tablets.
