CAJ | 학술논문

建立了顶空采样-毛细管气相色谱检测格列美脲原料药中溶剂残留的分析方法.对产自国内8个生产厂家的格列美脲样品中有机溶剂的残留状况进行了系统评价,结合药品生产工艺信息,确定了丙酮、乙酸乙酯、甲醇、异丙醇、乙醇、氯仿、甲苯、1,4-二氧六环、吡啶、氯苯、乙醚、二氯甲烷、正己烷和苯等14种有机溶剂为残留检测对象.根据被测组分在色谱柱上的保留性质将其分为两类,以实现基线分离.用Supelco-Wax极性色谱柱,以乙腈为内标物,分离检测了丙酮、乙酸乙酯、甲醇、异丙醇、乙醇、氯仿、甲苯、1,4-二氧六环、吡啶和氯苯的残留量;用Supelco OVI-G43弱极性色谱柱,以丁酮为内标,分离检测了乙醚、二氯甲烷、正己烷和苯的残留量.14种残留组分在各自的浓度范围内呈良好的线性关系(r=0.991 67～0.999 97,n=8),最低检出限范围为0.2～13.5 μg/g;14种残留组分检测的日间重复性(以相对标准偏差(RSD)计)为0.6% ～9.2%(n=3),3种加标浓度的平均添加回收率为86.3% ～104.1%(RSD为0.2% ～5.3%,n=16).实验结果表明,该方法简单、灵敏、可靠,适用于格列美脲中残留溶剂的分析确证.
건립료정공채양-모세관기상색보검측격렬미뇨원료약중용제잔류적분석방법.대산자국내8개생산엄가적격렬미뇨양품중유궤용제적잔류상황진행료계통평개,결합약품생산공예신식,학정료병동、을산을지、갑순、이병순、을순、록방、갑분、1,4-이양륙배、필정、록분、을미、이록갑완、정기완화분등14충유궤용제위잔류검측대상.근거피측조분재색보주상적보류성질장기분위량류,이실현기선분리.용Supelco-Wax겁성색보주,이을정위내표물,분리검측료병동、을산을지、갑순、이병순、을순、록방、갑분、1,4-이양륙배、필정화록분적잔류량;용Supelco OVI-G43약겁성색보주,이정동위내표,분리검측료을미、이록갑완、정기완화분적잔류량.14충잔류조분재각자적농도범위내정량호적선성관계(r=0.991 67～0.999 97,n=8),최저검출한범위위0.2～13.5 μg/g;14충잔류조분검측적일간중복성(이상대표준편차(RSD)계)위0.6% ～9.2%(n=3),3충가표농도적평균첨가회수솔위86.3% ～104.1%(RSD위0.2% ～5.3%,n=16).실험결과표명,해방법간단、령민、가고,괄용우격렬미뇨중잔류용제적분석학증.
An analytical method for the solvent residues in the raw material drug of glimepiride has been established by head-space sampling capillary gas chromatography. General evaluation was made for the distribution of organic residual solvents in glimepiride samples from 8 differ-ent domestic manufacturers. Based on the evaluation-test results and the information provided by manufacturers, 14 target solvents were ascertained including acetone, ethyl acetate, metha-nol, isopropanol, ethanol, chloroform, toluene, 1,4-dioxane, pyridine, chlorobenzene, ether, dichloromethane, n-hexane and benzene. The target solvents were divided into two groups for baseline separation according to their column-retention specificity. Acetone, ethyl acetate, methanol, isopropanol, ethanol, chloroform, toluene, 1,4-dioxane, pyridine and chloroben-zene were separated on a Supelco-Wax capillary column with acetonitrile as internal standard, while ether, dichloromethane, n-hexane and benzene were determined on a Supelco OVI-G43 capillary column with butanone as internal standard. Linear responses were obtained for the 14 residual solvents in their respective concentration ranges (r = 0. 991 67 - 0. 999 97 , n = 8), and the limits of detection were 0. 2-13. 5 μg/g. The inter-day reproducibilities, measured as rela-tive standard deviations (RSDs), were 0. 6%-9. 2% (n = 3). The average recoveries of three concentration levels were 86. 3%-104. 1% with the RSD of 0. 2% -5. 3% (N = 16). The devel-oped method is simple, sensitive, and accurate for the residual solvent analysis in glimepiride samples.