浙江农业学报
浙江農業學報
절강농업학보
ACTA AGRICULTURAE ZHEJIANGENSIS
2010年
1期
81-86
,共6页
铜%十二烷基硫酸钠%中华大蟾蜍蝌蚪%急性毒性%联合毒性%Na~+-K~+-ATP酶
銅%十二烷基硫痠鈉%中華大蟾蜍蝌蚪%急性毒性%聯閤毒性%Na~+-K~+-ATP酶
동%십이완기류산납%중화대섬서과두%급성독성%연합독성%Na~+-K~+-ATP매
copper%SDS%Bufo gargarizans%acute toxicity%joint toxicity:Na~+-K~+-ATPase
以中华大蟾蜍(Bufo gargarizans)蝌蚪为试验材料,采用联合指数相加法,研究了阴离子表面活性剂十二烷基硫酸钠(SDS)、重金属Cu~(2+)对蟾蜍蝌蚪的急性毒性和联合毒性效应;并在急性毒性试验的基础上,研究了亚急性条件下SDS,Cu~(2+)及其复合污染对蝌蚪肝脏Na~+-K~+-ATPase活性的影响.试验结果表明,SDS对蝌蚪的48,72,96 h的半致死浓度(LC_(50))分别为27.38,26.45,25.57 mg/L;Cu~(2+)对蝌蚪的48,72,96 h的半致死浓度(LC_(50))分别为0.475,0.323,0.285 ms/L.SDS,Cu~(2+)对蝌蚪的联合毒性作用48 h内表现为毒性增强的协同效应,96 h后表现为拮抗作用.蝌蚪在亚急性浓度的SDS,Cu~(2+)条件下暴露4 d后,SDS,Cu~(2+)及复合污染均对蝌蚪肝脏的Na~+-K~+-ATPase活性产生明显影响,SDS,Cu~(2+)复合污染在Na~+-K~+-ATPase水平上表现为一定的协同作用.
以中華大蟾蜍(Bufo gargarizans)蝌蚪為試驗材料,採用聯閤指數相加法,研究瞭陰離子錶麵活性劑十二烷基硫痠鈉(SDS)、重金屬Cu~(2+)對蟾蜍蝌蚪的急性毒性和聯閤毒性效應;併在急性毒性試驗的基礎上,研究瞭亞急性條件下SDS,Cu~(2+)及其複閤汙染對蝌蚪肝髒Na~+-K~+-ATPase活性的影響.試驗結果錶明,SDS對蝌蚪的48,72,96 h的半緻死濃度(LC_(50))分彆為27.38,26.45,25.57 mg/L;Cu~(2+)對蝌蚪的48,72,96 h的半緻死濃度(LC_(50))分彆為0.475,0.323,0.285 ms/L.SDS,Cu~(2+)對蝌蚪的聯閤毒性作用48 h內錶現為毒性增彊的協同效應,96 h後錶現為拮抗作用.蝌蚪在亞急性濃度的SDS,Cu~(2+)條件下暴露4 d後,SDS,Cu~(2+)及複閤汙染均對蝌蚪肝髒的Na~+-K~+-ATPase活性產生明顯影響,SDS,Cu~(2+)複閤汙染在Na~+-K~+-ATPase水平上錶現為一定的協同作用.
이중화대섬서(Bufo gargarizans)과두위시험재료,채용연합지수상가법,연구료음리자표면활성제십이완기류산납(SDS)、중금속Cu~(2+)대섬서과두적급성독성화연합독성효응;병재급성독성시험적기출상,연구료아급성조건하SDS,Cu~(2+)급기복합오염대과두간장Na~+-K~+-ATPase활성적영향.시험결과표명,SDS대과두적48,72,96 h적반치사농도(LC_(50))분별위27.38,26.45,25.57 mg/L;Cu~(2+)대과두적48,72,96 h적반치사농도(LC_(50))분별위0.475,0.323,0.285 ms/L.SDS,Cu~(2+)대과두적연합독성작용48 h내표현위독성증강적협동효응,96 h후표현위길항작용.과두재아급성농도적SDS,Cu~(2+)조건하폭로4 d후,SDS,Cu~(2+)급복합오염균대과두간장적Na~+-K~+-ATPase활성산생명현영향,SDS,Cu~(2+)복합오염재Na~+-K~+-ATPase수평상표현위일정적협동작용.
To investigate the effects of single or combined sodium dodeeyl sulfate(SDS)and Cu~(2+) pollution in Bufo gargartzans,the acute toxicity and joint toxicity tests of SDS,Cu~(2+) and Na~+-K~+-ATPase activity in liver under the subacute toxicities of single or combined SDS and Cu~(2+) were measured by using exposare experiment.The results showed that the Lc_(50) of SDS for 48,72 and 96 h to Bufo gargarizans were 27.38,26.45 and 25.57 mg·L~(-1).respectively,and the LC_(50) of Cu~(2+) for 48,72 and 96 h were0.475,0.323 and 0.285 mg·L~(-1),respectively.The toxieity of Cu~(2+) was higher than that of SDS.In addition,it also indicated that the joint toxicity of Cu~(2+)and SDS was synergistic at 48 h but antagonistic at 96 h.The results also indicated that single or combined treatments with Cu~(2+) and SDS could affect the Na~+-K~+-ATPase activity in liver of Bufo gargarizans,and the joint effect of the eombined pollution on the Na~+-K~+-ATPase activity Was synergistic.
