CAJ | 학술논문

[目的]研究细胞内可溶性物质浓度、活性氧自由基清除能力对东乡野生稻耐寒性的生理影响.[方法]以东乡野生稻为材料,同时以2种普通栽培稻协青早B和农大288为对照,对比检测它们在各个生长时期叶片中的可溶性糖含量、游离脯氨酸含量、可溶性蛋白含量、POD活性、CAT活性、丙二醛(MDA)含量.可溶性糖含量测定采用苯酚-浓硫酸比色定糖法;游离脯氨酸含量测定采用茚三酮显色法;可溶性蛋白含量测定采用考马斯亮蓝法;过氧化物酶(POD)活力测定采用愈创木酚法;过氧化氢酶(CAT)活性测定采用硫代硫酸钠滴定法;MDA含量测定采用硫代巴比妥酸法.[结果]在各个生长时期东乡野生稻叶片可溶性蛋白、可溶性糖和游离脯氨酸含量均维持在较高的浓度.这些可溶性物质含量的高低对于植物抗逆性具有重要的意义,它们可能通过提高或维持细胞质的溶质浓度,来达到增强植株抗性的目的;在冬季,东乡野生稻叶片中POD活性水平高,有效地清除叶片细胞中由低温激发产生的活性氧自由基,从而起到保护细胞的作用;而其CAT活性低温条件下明显降低,这可能与POD与CAT的结构和作用机理不同有关;叶片组织中丙二醛含量波动不大,较对照水稻品种高.[结论]该研究为进一步探讨东乡野生稻耐寒生理的分子机理奠定了基础.
[목적]연구세포내가용성물질농도、활성양자유기청제능력대동향야생도내한성적생리영향.[방법]이동향야생도위재료,동시이2충보통재배도협청조B화농대288위대조,대비검측타문재각개생장시기협편중적가용성당함량、유리포안산함량、가용성단백함량、POD활성、CAT활성、병이철(MDA)함량.가용성당함량측정채용분분-농류산비색정당법;유리포안산함량측정채용인삼동현색법;가용성단백함량측정채용고마사량람법;과양화물매(POD)활력측정채용유창목분법;과양화경매(CAT)활성측정채용류대류산납적정법;MDA함량측정채용류대파비타산법.[결과]재각개생장시기동향야생도협편가용성단백、가용성당화유리포안산함량균유지재교고적농도.저사가용성물질함량적고저대우식물항역성구유중요적의의,타문가능통과제고혹유지세포질적용질농도,래체도증강식주항성적목적;재동계,동향야생도협편중POD활성수평고,유효지청제협편세포중유저온격발산생적활성양자유기,종이기도보호세포적작용;이기CAT활성저온조건하명현강저,저가능여POD여CAT적결구화작용궤리불동유관;협편조직중병이철함량파동불대,교대조수도품충고.[결론]해연구위진일보탐토동향야생도내한생리적분자궤리전정료기출.
[Objective] The research aimed to study the effects of soluble substance concentration in cell and the scavenging activity of active oxygen radicals on the cold-tolerance physiology of Dongxiang wild rice. [Method] Dongxiang wild rice was used as materials to detect and compare the content of soluble sugar, free proline and soluble protein, POD activity, CAT activity, MDA content in the leaves in different growth periods.Using with control of two species of common cultivated rice Xieqingzao and Nongda 288. [Result] The contents of soluble protein, soluble sugar, and free proline in the leaves of Dongxiang wild rice were higher in the whole growth periods. POD activity maintained at high level. The content of MDA in the leaf tissues in Dongxiang wild rice was had no great fluctuation and it was relative higher than that in control rice varieties. [Conclusion] This study laid the foundation for further insight into the physiological mechanism of the cold-tolerance of Dongxiang wild rice.