CAJ | 학술논문

目的探讨Nogo-A蛋白在少突胶质细胞肿瘤中的表达水平与该肿瘤恶性程度之间的关系.方法选取来自临床上经病理检查确诊为少突胶质细胞肿瘤的组织标本,其中少突胶质细胞瘤16例,间变型少突胶质细胞瘤10例.免疫组织化学染色检测Nogo-A的表达,并与同视野下瘤细胞的核分裂与核异型性比例作相关分析;另外,把病理标本按照分级进行蛋白印迹试验.结果免疫组织化学结果显示,Nogo-A免疫阳性染色的光密度值与肿瘤细胞核分裂、核异型比例呈明显的负相关,对蛋白印迹试验结果进行光密度分析也发现少突胶质细胞瘤中的Nogo-A蛋白条带灰度值明显高于间变型少突胶质细胞瘤中的灰度值.结论 Nogo-A蛋白在少突胶质细胞肿瘤中的表达水平与该肿瘤恶性程度呈负相关.
목적 탐토Nogo-A단백재소돌효질세포종류중적표체수평여해종류악성정도지간적관계.방법 선취래자림상상경병리검사학진위소돌효질세포종류적조직표본,기중소돌효질세포류16례,간변형소돌효질세포류10례.면역조직화학염색검측Nogo-A적표체,병여동시야하류세포적핵분렬여핵이형성비례작상관분석;령외,파병리표본안조분급진행단백인적시험.결과 면역조직화학결과현시,Nogo-A면역양성염색적광밀도치여종류세포핵분렬、핵이형비례정명현적부상관,대단백인적시험결과진행광밀도분석야발현소돌효질세포류중적Nogo-A단백조대회도치명현고우간변형소돌효질세포류중적회도치.결론 Nogo-A단백재소돌효질세포종류중적표체수평여해종류악성정도정부상관.
Objective Nogo-A is an axon regeneration inhibitor, and its function in central nervous system (CNS) is still unknown. The present study is to explore the relationship between the expression of Nogo-A and the malignancy of oligodendroglial tumors in patients. Methods Tumor tissue samples with different malignancy grade were obtained from the hospitals. The samples used for detection had been diagnosed as oligodendroglial tumors (oligodendroglioma or anaplastic oligodendroglioma). The expression of Nogo-A was detected by immunohistochemistry and western-blot analysis. The correlation test between the Nogo-A expression and the morphological changes (the percentages of atypical cells and mitotic cells in the tumors) related to the malignancy of tumor tissues was performed. Results There was significant negative correlation between the Nogo-A expression and the morphological change of tumor tissues according to immunohistochemistry. Western-blot analysis also indicated that the gray value of Nogo-A protein band in the oligodendroglioma group was significantly higher than that in the anaplastic oligodendroglioma group. Conclusion Nogo-A expression was negatively correlated with the malignancy grade of oligodendroglial tumors.