国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2012年
6期
384-387,封3
,共5页
邱谢武%于聪慧%王剑飞%奈超%田玉旺
邱謝武%于聰慧%王劍飛%奈超%田玉旺
구사무%우총혜%왕검비%내초%전옥왕
肝癌%肝癌干细胞%CD13%免疫组织化学
肝癌%肝癌榦細胞%CD13%免疫組織化學
간암%간암간세포%CD13%면역조직화학
Hepatocellular carcinoma%Liver cancer stem cells%CD13%Immunohistochemistry
目的 探讨氨肽酶N/CD13在肝癌患者中的表达情况,探讨其与肝癌患者临床资料及预后的相关性.方法 应用免疫组化SP法,对40例肝癌患者肝癌组织、10例肝癌患者癌旁组织及10例肝血管瘤患者正常肝组织行单克隆抗体CD13检测.结果 40例(100％)肝癌组织均可见不同程度的CD13阳性表达,肝癌癌旁组织中3例(30％)出现CD13弱阳性表达,10例肝血管瘤患者标本未见表达.CD13表达量与患者的性别、年龄、血清AFP值、HbsAg、分化程度、Child分级及TNM分期差异无统计学意义(P＞0.05)；而CD13的表达量与患者的血清AFP值、HbsAg、分化程度之间差异有统计学意义(P＜0.05).生存函数图发现CD13与患者生存时间呈反相关,但CD13表达量与患者肿瘤复发差异无统计学意义(P＞0.05).结论 CD13可作为肝癌干细胞的表面标志物,有望成为判断预后的有效指标.
目的 探討氨肽酶N/CD13在肝癌患者中的錶達情況,探討其與肝癌患者臨床資料及預後的相關性.方法 應用免疫組化SP法,對40例肝癌患者肝癌組織、10例肝癌患者癌徬組織及10例肝血管瘤患者正常肝組織行單剋隆抗體CD13檢測.結果 40例(100％)肝癌組織均可見不同程度的CD13暘性錶達,肝癌癌徬組織中3例(30％)齣現CD13弱暘性錶達,10例肝血管瘤患者標本未見錶達.CD13錶達量與患者的性彆、年齡、血清AFP值、HbsAg、分化程度、Child分級及TNM分期差異無統計學意義(P＞0.05)；而CD13的錶達量與患者的血清AFP值、HbsAg、分化程度之間差異有統計學意義(P＜0.05).生存函數圖髮現CD13與患者生存時間呈反相關,但CD13錶達量與患者腫瘤複髮差異無統計學意義(P＞0.05).結論 CD13可作為肝癌榦細胞的錶麵標誌物,有望成為判斷預後的有效指標.
목적 탐토안태매N/CD13재간암환자중적표체정황,탐토기여간암환자림상자료급예후적상관성.방법 응용면역조화SP법,대40례간암환자간암조직、10례간암환자암방조직급10례간혈관류환자정상간조직행단극륭항체CD13검측.결과 40례(100％)간암조직균가견불동정도적CD13양성표체,간암암방조직중3례(30％)출현CD13약양성표체,10례간혈관류환자표본미견표체.CD13표체량여환자적성별、년령、혈청AFP치、HbsAg、분화정도、Child분급급TNM분기차이무통계학의의(P＞0.05)；이CD13적표체량여환자적혈청AFP치、HbsAg、분화정도지간차이유통계학의의(P＜0.05).생존함수도발현CD13여환자생존시간정반상관,단CD13표체량여환자종류복발차이무통계학의의(P＞0.05).결론 CD13가작위간암간세포적표면표지물,유망성위판단예후적유효지표.
Objective To study aminopeptidase N/CD13 expression in hepatocellular carcinoma and its relationship with clinical data and proguosis in patients with hepatocellular carcinoma.Methods The immunohistochemical SP method was used to detect the CD13 monoclonal antibody in 40 cases of hepatocellular carcinoma,10 cases of corresponding para-carcinoma and 10 cases of cavernous hemangioma.Results Forty cases ( 100％ ) hepatocellular carcinoma tissues were seen varying degrees of CD13 expression,3 (30％) corresponding para-carcinoma tissues were weakly positive,and 10 cases of cavernous hemangioma with no expression.The expression rate of CD13 was not significantly correlated with the patient gender,age,serum AFP value,HbsAg,differentiation,CHILD grade and TNM stage (P＞0.05).But the expression of CD13 was closely related with the patient serum AFP value,HbsAg,differentiation ( P ＜ 0.05 ).By the survival function graph we could find the expression rate was negativly correlated with survival in patients,but the expression was not significantly correlated with tumor relapse.Conclusion CD13 can be used as the surface marker of liver cancer stem cells,and it is expected to become an effective indicator of prognosis in patients with hepatocellular carcinoma.
