国际脑血管病杂志
國際腦血管病雜誌
국제뇌혈관병잡지
INTERNATIONAL JOURNAL OF CEREBROVASCULAR DISEASES
2011年
3期
220-225
,共6页
张昆南%刘世民%胡国柱%熊英琼%龚凌云%胡凡%柴文%吴晓牧
張昆南%劉世民%鬍國柱%熊英瓊%龔凌雲%鬍凡%柴文%吳曉牧
장곤남%류세민%호국주%웅영경%공릉운%호범%시문%오효목
脑缺血%磷酸丙酮酸水合酶%胶质纤维酸性蛋白%突触囊泡蛋白%Nogo蛋白%时间因素%疾病模型,动物%大鼠
腦缺血%燐痠丙酮痠水閤酶%膠質纖維痠性蛋白%突觸囊泡蛋白%Nogo蛋白%時間因素%疾病模型,動物%大鼠
뇌결혈%린산병동산수합매%효질섬유산성단백%돌촉낭포단백%Nogo단백%시간인소%질병모형,동물%대서
Brain ischemia%Phosphopyruvate hydratase%Glial fibrillary acidic protein%Synaptophysin%Nogo protein%Time factors%Disease models,animal%Rats
目的 探讨脑缺血再灌注恢复期大鼠梗死周围组织胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、神经元特异性烯醇化酶(neuron-specific enolase,NSE)、突触素(synaptophysin,SYN)、神经突生长抑制因子-A(neurite outgowth inhibito-A,Nogo-A)表达与神经功能转归的相关性.方法 线栓法制作大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)2 h再灌注模型,模型制作后28、35、42和49 d改良神经功能缺损评分(modified neurological severity score,mNSS),并采用免疫组化方法检测脑梗死周围组织GFAP、NSE、SYN、Nogo-A表达.结果 大鼠脑缺血再灌注后随着时间推移,mNSS评分逐渐下降,除第35天(5.11±0.737)与第42天(4.54±0.519)、第42天与第49天(4.29±0.488)无显著差异外,其余各时间点均有显著差异(P均<0.05).GFAP阳性细胞数量从第28天至第49天逐渐减少,其中第42天(51.00±13.59)和第49天(44.38±11.94)显著少于第28天(69.00±15.10)(P<0.05).NSE表达的累积光密度(integrated optical density,IOD)逐渐增高.第28天(6 218.57±1 864.25)与第42(9 414.00±2 491.12)和第49天(12 522.50 ±3 106.99),第35天(7 343.40±1533.35)与第49天差异显著(P均<0.05),其余各时间点无显著差异.SYN表达IOD逐渐增高,第49天(66 503.00±12 834.61)显著高于第28天(43 905.14 ±13 208.59)(P<0.05).Nogo-A阳性细胞数量逐渐减少,第49天(42.13±14.45)显著少于第28天(59.57±15.25)(P<0.05).GFAP表达与mNSSn评分呈正相关(r=0.993,P=0.007),NSE(r=-0.954,P=0.044)、SYN(r=-0.992,P=0.008)表达与mNSS评分呈负相关.结论 大鼠脑缺血再灌注后恢复期神经功能转归与GFAP表达下调、NSE和SYN表达上调相关.
目的 探討腦缺血再灌註恢複期大鼠梗死週圍組織膠質纖維痠性蛋白(glial fibrillary acidic protein,GFAP)、神經元特異性烯醇化酶(neuron-specific enolase,NSE)、突觸素(synaptophysin,SYN)、神經突生長抑製因子-A(neurite outgowth inhibito-A,Nogo-A)錶達與神經功能轉歸的相關性.方法 線栓法製作大腦中動脈閉塞(middle cerebral artery occlusion,MCAO)2 h再灌註模型,模型製作後28、35、42和49 d改良神經功能缺損評分(modified neurological severity score,mNSS),併採用免疫組化方法檢測腦梗死週圍組織GFAP、NSE、SYN、Nogo-A錶達.結果 大鼠腦缺血再灌註後隨著時間推移,mNSS評分逐漸下降,除第35天(5.11±0.737)與第42天(4.54±0.519)、第42天與第49天(4.29±0.488)無顯著差異外,其餘各時間點均有顯著差異(P均<0.05).GFAP暘性細胞數量從第28天至第49天逐漸減少,其中第42天(51.00±13.59)和第49天(44.38±11.94)顯著少于第28天(69.00±15.10)(P<0.05).NSE錶達的纍積光密度(integrated optical density,IOD)逐漸增高.第28天(6 218.57±1 864.25)與第42(9 414.00±2 491.12)和第49天(12 522.50 ±3 106.99),第35天(7 343.40±1533.35)與第49天差異顯著(P均<0.05),其餘各時間點無顯著差異.SYN錶達IOD逐漸增高,第49天(66 503.00±12 834.61)顯著高于第28天(43 905.14 ±13 208.59)(P<0.05).Nogo-A暘性細胞數量逐漸減少,第49天(42.13±14.45)顯著少于第28天(59.57±15.25)(P<0.05).GFAP錶達與mNSSn評分呈正相關(r=0.993,P=0.007),NSE(r=-0.954,P=0.044)、SYN(r=-0.992,P=0.008)錶達與mNSS評分呈負相關.結論 大鼠腦缺血再灌註後恢複期神經功能轉歸與GFAP錶達下調、NSE和SYN錶達上調相關.
목적 탐토뇌결혈재관주회복기대서경사주위조직효질섬유산성단백(glial fibrillary acidic protein,GFAP)、신경원특이성희순화매(neuron-specific enolase,NSE)、돌촉소(synaptophysin,SYN)、신경돌생장억제인자-A(neurite outgowth inhibito-A,Nogo-A)표체여신경공능전귀적상관성.방법 선전법제작대뇌중동맥폐새(middle cerebral artery occlusion,MCAO)2 h재관주모형,모형제작후28、35、42화49 d개량신경공능결손평분(modified neurological severity score,mNSS),병채용면역조화방법검측뇌경사주위조직GFAP、NSE、SYN、Nogo-A표체.결과 대서뇌결혈재관주후수착시간추이,mNSS평분축점하강,제제35천(5.11±0.737)여제42천(4.54±0.519)、제42천여제49천(4.29±0.488)무현저차이외,기여각시간점균유현저차이(P균<0.05).GFAP양성세포수량종제28천지제49천축점감소,기중제42천(51.00±13.59)화제49천(44.38±11.94)현저소우제28천(69.00±15.10)(P<0.05).NSE표체적루적광밀도(integrated optical density,IOD)축점증고.제28천(6 218.57±1 864.25)여제42(9 414.00±2 491.12)화제49천(12 522.50 ±3 106.99),제35천(7 343.40±1533.35)여제49천차이현저(P균<0.05),기여각시간점무현저차이.SYN표체IOD축점증고,제49천(66 503.00±12 834.61)현저고우제28천(43 905.14 ±13 208.59)(P<0.05).Nogo-A양성세포수량축점감소,제49천(42.13±14.45)현저소우제28천(59.57±15.25)(P<0.05).GFAP표체여mNSSn평분정정상관(r=0.993,P=0.007),NSE(r=-0.954,P=0.044)、SYN(r=-0.992,P=0.008)표체여mNSS평분정부상관.결론 대서뇌결혈재관주후회복기신경공능전귀여GFAP표체하조、NSE화SYN표체상조상관.
Objective To investigate the correlation between the glial fibrillary acidic protein(GFAP),neuron-specific enolase(NSE),synaptoghysin (SYN),neurite outgrowth inhibitor-A(Nogo-A)expression and neurological outcome in tissue surrounding the infarct during the recovery after cerebral ischemia-reperfusion injury in rats.Methods A 2-hour middle cerebral artery occlusion(MCAO)and reperfusion model in rats was induced by the intraluminal suture method.The modified neurological severity score(mNSS)was performed at day 28,35,42,and 49.Immunohistochemistry was used to detect the expressions of GFAP,NSE,SYN,and Noga-A in tissue surrounding the infarct.Results The mNSS score decreased gradually over time after cerdnal ischemia-reperfusion injury in rats.Except day 35(5.11±0.737)vs.day 42 (4.54±0.519),and day 42 vs.day 49(4.29±0.488),there were significant differences at all other time points(all P<0.05).The numbers of GFAP positive cells deergased gradually form day 28 to day 49,in which,the numbers of GFAP positive cells at day 42(51.00±13.59)vs.day 49(44.38±11.94) were significantly less than those at day 28(69.00±15.10)(P<0.05).There were no significant differences in the numbers of NSE positive cells at all time points,but their integrated optical density(IOD)increased gradually.There were significant differences between day 28(6 218.57±1 864.25)and day 42(9 414.00±2 491.12) or day 49(12 522.50±3 106.99),and between day 35(7 343.40±1 533.35)and day 49(all P< 0.05).There were no significant differences at all other time points.The SYN express (IOD)increased gradually.and it was significantly lower at day 49(66 503.00±12 834.61)than that at day 28(43 905.14±13 208.59)(P<0.05).The numbers of Nogo-A positive cells decreased gradually,and they were significantly less at day 49(42.13±14.45) than those at day 28(59.57±15.25)(P<0.05).The GFAP expression was positively correlated with the mNSS scores(r=0.993,P=0.007).The NSE(r=-0.954,P=0.044)and SYN(r=-0.992,P=0.008) expression was negatively correlated with the mNSS scores.Conclusion The neurological outcome was associated with the downregulation of GFAP expression and the upregalation of NSE and SYN expression during the recovery after cerebral ischemia-reperfusion injury in rats.
