CAJ | 학술논문

目的对家族聚集性高血压心血管疾病标志物功能分类基因表达进行研究,以筛查候选基因.方法选择3代以上直系亲属均有原发性高血压病史的患者作为实验组,并以健康志愿者作为对照组.采用Oligo GEArray基因芯片技术检测外周血液心血管疾病标志物基因的表达,以阳性标准值/阴性标准值＞2.0,或≤0.5和＞0的基因为差异表达基因.结果与对照组相比,实验组心血管疾病标志物功能分类基因表达上调的基因共有10条,涉及脂质代谢、免疫应答相关分子、细胞黏附分子、细胞外分子及凝血基因,包括载脂蛋白E(ApoE)、上皮V样抗原-1(EVA-1)、γ-干扰素(IFN-γ)、白细胞介素-1β(IL-1β)、IL-8、整连蛋白-β(ITGB-1)、基质金属蛋白酶-9(MMP-9)、核转录因子-κB(NF-κB)、血小板-内皮细胞黏附分子-1(PECAM-1)、P-选择素(SEL-P);下调基因3条,包括凝血因子-Ⅲ(F-Ⅲ)、血凝素样氧化型低密度脂蛋白受体-1(LOX-1)、丝氨酸蛋白酶抑制因子-1(SERPINE-1).结论家族聚集性高血压涉及多种心血管疾病标志物基因,尤其与凝血相关的F-Ⅲ和与细胞外分子蛋白酶抑制剂、凝血相关的SERPINE-1两个基因有关.
목적 대가족취집성고혈압심혈관질병표지물공능분류기인표체진행연구,이사사후선기인.방법 선택3대이상직계친속균유원발성고혈압병사적환자작위실험조,병이건강지원자작위대조조.채용Oligo GEArray기인심편기술검측외주혈액심혈관질병표지물기인적표체,이양성표준치/음성표준치＞2.0,혹≤0.5화＞0적기인위차이표체기인.결과 여대조조상비,실험조심혈관질병표지물공능분류기인표체상조적기인공유10조,섭급지질대사、면역응답상관분자、세포점부분자、세포외분자급응혈기인,포괄재지단백E(ApoE)、상피V양항원-1(EVA-1)、γ-간우소(IFN-γ)、백세포개소-1β(IL-1β)、IL-8、정련단백-β(ITGB-1)、기질금속단백매-9(MMP-9)、핵전록인자-κB(NF-κB)、혈소판-내피세포점부분자-1(PECAM-1)、P-선택소(SEL-P);하조기인3조,포괄응혈인자-Ⅲ(F-Ⅲ)、혈응소양양화형저밀도지단백수체-1(LOX-1)、사안산단백매억제인자-1(SERPINE-1).결론 가족취집성고혈압섭급다충심혈관질병표지물기인,우기여응혈상관적F-Ⅲ화여세포외분자단백매억제제、응혈상관적SERPINE-1량개기인유관.
Objective To explore the cardiovascular diseases marker gene expression profile of the familial aggregation hypertension patients,and to screen differentially expressed genes.Methods The patients who had directly related family members for more than three generations suffering from hypertension were selected as experiment group, and healthy individuals as control group.Oligo GEArray gene chip technique was used to detect the expression of cardiovascular diseases marker gene in peripheral blood.The ratio of positive/negative standard value ＞2.0, or ≤0.5 and ＞0 was indentified as differential gene.Results Compared with control group,there were 10 up-regulated differential genes in experiment group, composing genes involved in lipid metabolism, immune response-related molecules, cell adhesion molecules, extracellular molecules and coagulation,including apolipoprotein E (ApoE),epithelial V-like antigen-1 (EVA-1),interferon-γ (IFN-γ), interleukin-1β (IL-1β), IL-8, integrin-β1 (ITGB-1), matrix metalloproteinase-9 (MMP-9), uclear factor-κB (NF-κB), platelet endothelial cell adhesion molecule-1 (PECAM-1),selectin-P(SEL-P).There were 3 down-regulated genes, including coagulation factors- Ⅲ (F- Ⅲ ), lectin-like oxidized low density lipoprotein receptor-1 (LOX-1), and serine protease inhibitor-1 (SERPINE-1).Conclusion This study suggested that familial aggregation hypertension related to a variety of gene markers of cardiovascular disease, especially elements concerning coagulation and extracellular protease inhibitor-related genes.