CAJ | 학술논문

目的探讨红细胞生成素(EPO)对慢性肾衰竭大鼠外周血内皮祖细胞(EPC)数量和功能的影响.方法采用分阶段5/6肾切除制备大鼠慢性肾衰竭模型.实验动物按数字随机表法分为4组(均n=7):假手术组、慢性肾衰竭组(模型组)、30 U/kg EPO干预组(小剂量组)和50 U/kg EPO干预组(大剂量组).大鼠皮下注射EPO 6周后,取其外周血分离培养EPC,并检测EPC数量及其增殖、黏附和形成血管结构的能力.结果与假手术组比较,慢性肾衰竭大鼠外周血EPC数量及其增殖、黏附与形成血管结构的能力均显著下降(均P＜0.05).应用EPO治疗能显著增加慢性肾衰竭大鼠外周血EPC数量(P＜0.05),改善外周血EPC增殖、黏附及形成血管结构的能力(均P＜0.05),并且呈剂量依赖性.结论 EPO可改善慢性肾衰竭大鼠外周血EPC的数量和功能.
목적 탐토홍세포생성소(EPO)대만성신쇠갈대서외주혈내피조세포(EPC)수량화공능적영향.방법 채용분계단5/6신절제제비대서만성신쇠갈모형.실험동물안수자수궤표법분위4조(균n=7):가수술조、만성신쇠갈조(모형조)、30 U/kg EPO간예조(소제량조)화50 U/kg EPO간예조(대제량조).대서피하주사EPO 6주후,취기외주혈분리배양EPC,병검측EPC수량급기증식、점부화형성혈관결구적능력.결과 여가수술조비교,만성신쇠갈대서외주혈EPC수량급기증식、점부여형성혈관결구적능력균현저하강(균P＜0.05).응용EPO치료능현저증가만성신쇠갈대서외주혈EPC수량(P＜0.05),개선외주혈EPC증식、점부급형성혈관결구적능력(균P＜0.05),병차정제량의뢰성.결론 EPO가개선만성신쇠갈대서외주혈EPC적수량화공능.
Objective To investigate the effects of erythropoietin (EPO) on the number and function of peripheral blood endothelial progenitor cells (EPCs) from rats with chronic renal failure (CRF). Methods The model of chronic renal failure was established by a two-stage 5/6nephrectomy procedure in rats. Experimental rats were randomly divided into four groups (n =7,respectively): sham operation group, CRF group, CRF rats treated with 30 U/kg EPO (low-dosage group) and with 50 U/kg EPO (high-dosage group). CRF rats were given EPO by hypodermic injection for 6 weeks, then EPCs were isolated by density gradient centrifugation from peripheral blood mononuclear cells. The ability of cell proliferation, adhesion and vasculogenesis in vitro was further observed. Results Compared to sham operation group, the ability of cell proliferation,adhesion and vasculogenesis in vitro in CRF rats was remarkably decreased (P＜0.05, respectively).Such ability was promoted significantly in dose-dependent manner by EPO treatment (P＜0.05,respectively). Conclusion EPO can improve the number and ability of endothelial progenitor cells from rats with chronic renal failure.