中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
7期
855-856
,共2页
锁涛%张涛%童赛雄%艾志龙%刘厚宝%秦新裕%胡美玉%王志军
鎖濤%張濤%童賽雄%艾誌龍%劉厚寶%秦新裕%鬍美玉%王誌軍
쇄도%장도%동새웅%애지룡%류후보%진신유%호미옥%왕지군
胆囊癌%格尔德霉素%转移
膽囊癌%格爾德黴素%轉移
담낭암%격이덕매소%전이
Gallbladder carcinoma%Geldanamycin%Metastasis
目的 观察格尔德霉素(GA)对胆囊癌细胞生长、运动和侵袭的影响及其化疗增敏作用.方法 噻唑蓝(MTT)比色法检测(0、0.0064、0.032、0.16、0.8、4、20、100)×10-6 mol/L浓度梯度的GA对胆囊癌细胞GBC-SD生长影响;观察1 μmoL/L的GA对0.1倍最大血药浓度(Maximal plasma concentration,Cmax)的5-氟尿嘧啶(5-Fu,Cmax 10 mg/L)、丝裂霉素(MMC,Cmax 3 mg/L)、阿霉素(ADM,Cmax 0.4 mg/L)的化疗增敏作用.应用迁移运动及侵袭实验观察1μmol/L的GA体外对胆囊癌细胞运动、侵袭能力的影响.结果 GA体外对胆囊癌细胞有较强的抑制作用,半数抑制浓度(Inhibitory concentration 50%,IC50)在20μmol/L水平.1μmol/L低生长抑制浓度的GA能够增加传统化疗药物对GBC-SD生长的抑制作用,有明显的增效作用;同时低浓度的GA可以抑制肝细胞生长因子(HGF)对GBC-SD运动迁移和侵袭能力的诱导作用.结论 GA有可能成为胆囊癌化疗和抗转移的有效药物.
目的 觀察格爾德黴素(GA)對膽囊癌細胞生長、運動和侵襲的影響及其化療增敏作用.方法 噻唑藍(MTT)比色法檢測(0、0.0064、0.032、0.16、0.8、4、20、100)×10-6 mol/L濃度梯度的GA對膽囊癌細胞GBC-SD生長影響;觀察1 μmoL/L的GA對0.1倍最大血藥濃度(Maximal plasma concentration,Cmax)的5-氟尿嘧啶(5-Fu,Cmax 10 mg/L)、絲裂黴素(MMC,Cmax 3 mg/L)、阿黴素(ADM,Cmax 0.4 mg/L)的化療增敏作用.應用遷移運動及侵襲實驗觀察1μmol/L的GA體外對膽囊癌細胞運動、侵襲能力的影響.結果 GA體外對膽囊癌細胞有較彊的抑製作用,半數抑製濃度(Inhibitory concentration 50%,IC50)在20μmol/L水平.1μmol/L低生長抑製濃度的GA能夠增加傳統化療藥物對GBC-SD生長的抑製作用,有明顯的增效作用;同時低濃度的GA可以抑製肝細胞生長因子(HGF)對GBC-SD運動遷移和侵襲能力的誘導作用.結論 GA有可能成為膽囊癌化療和抗轉移的有效藥物.
목적 관찰격이덕매소(GA)대담낭암세포생장、운동화침습적영향급기화료증민작용.방법 새서람(MTT)비색법검측(0、0.0064、0.032、0.16、0.8、4、20、100)×10-6 mol/L농도제도적GA대담낭암세포GBC-SD생장영향;관찰1 μmoL/L적GA대0.1배최대혈약농도(Maximal plasma concentration,Cmax)적5-불뇨밀정(5-Fu,Cmax 10 mg/L)、사렬매소(MMC,Cmax 3 mg/L)、아매소(ADM,Cmax 0.4 mg/L)적화료증민작용.응용천이운동급침습실험관찰1μmol/L적GA체외대담낭암세포운동、침습능력적영향.결과 GA체외대담낭암세포유교강적억제작용,반수억제농도(Inhibitory concentration 50%,IC50)재20μmol/L수평.1μmol/L저생장억제농도적GA능구증가전통화료약물대GBC-SD생장적억제작용,유명현적증효작용;동시저농도적GA가이억제간세포생장인자(HGF)대GBC-SD운동천이화침습능력적유도작용.결론 GA유가능성위담낭암화료화항전이적유효약물.
Objective To study the effects of Geldanamycin (GA) on the proliferation, motility and invasion of gallbladder carcinoma (GBC) cells and its synergistic effects with S-Fu, ADM and MMC in vitro. Methods Methyl thiazolyl tetrazolium(MTT) assays were used to investigate the effects of GA [(0,0.0064,0.032,0.16,0.8,4,20, 100)×10-6 mol/L] on the proliferation of GBC-SD. The enhanced antitumor activities of 5-Fu (Cmax 10 mg/L) , MMC (Cmax 3 mg/L) and ADM (Cmax 0.4 mg/L) at 0.1 × Cmax were observed in combination with GA at 1 μmol/L (a very low cytotoxic concentration). Motility and invasion abilities were tested to evaluate the inhibitory effects of GA at 1 μmol/L. Results In MTT assays, GA could inhibit growth of cancer cells sharply at the concentration of 20 μmol/L (IC50). Although it couldn't postpone the motility and invasion apparently by itself, GA at 1 μmol/L could inhibit the HGF/SF-mediated increase of motility and invasion. The enhanced antitumor activity of 5-Fu, MMC and ADM was observed in combination with GA at 1 μmol/L The synergistic effects were very significant. Conclusion GA may be potent agents to inhibit development and metastasis of GBC in the near future.
