中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
7期
956-957,封3
,共3页
膀胱%盆神经节%神经递质%人工反射弧
膀胱%盆神經節%神經遞質%人工反射弧
방광%분신경절%신경체질%인공반사호
Bladder%Pelvic ganglion%Neurotransmitter%Artificial reflex arc
目的 观察人丁体神经-内脏神经反射弧建立后大鼠膀胱和盆神经节(MPG)之间的神经通路及神经递质性质.方法 8只成年wistar大鼠在体建立人工体神经-内脏神经反射弧.将辣根过氧化物酶(HRP)注射至实验大鼠的膀胱壁肌层,逆行神经追踪,通过免疫组织化学或组织化学方法显示HRP阳性标记细胞中的胆碱已酰转移酶(ChAT)、酪氨酸羟化酶(TH)、血管活性肠肽(VIP)、P物质(SP)和还原型辅酶Ⅱ(NADPH,NOS标志物).结果 支配膀胱的神经元多位于MPG主体部,呈圆形或椭圆形,无明显突起,多数为ChAT(40.1%,112/279)和TH(20.4%,50/245)免疫阳性,少数为VIP(10.7%,25/233)、NOS(6.5%,15/230)、SP(3.3%,8/242)反应阳性.结论 人工体神经-内脏神经反射弧建立后MPG中支配膀胱的神经元以胆碱能和肾上腺素能为主,VIP、SP和一氧化氮(NO)可能发挥神经调质作用.
目的 觀察人丁體神經-內髒神經反射弧建立後大鼠膀胱和盆神經節(MPG)之間的神經通路及神經遞質性質.方法 8隻成年wistar大鼠在體建立人工體神經-內髒神經反射弧.將辣根過氧化物酶(HRP)註射至實驗大鼠的膀胱壁肌層,逆行神經追蹤,通過免疫組織化學或組織化學方法顯示HRP暘性標記細胞中的膽堿已酰轉移酶(ChAT)、酪氨痠羥化酶(TH)、血管活性腸肽(VIP)、P物質(SP)和還原型輔酶Ⅱ(NADPH,NOS標誌物).結果 支配膀胱的神經元多位于MPG主體部,呈圓形或橢圓形,無明顯突起,多數為ChAT(40.1%,112/279)和TH(20.4%,50/245)免疫暘性,少數為VIP(10.7%,25/233)、NOS(6.5%,15/230)、SP(3.3%,8/242)反應暘性.結論 人工體神經-內髒神經反射弧建立後MPG中支配膀胱的神經元以膽堿能和腎上腺素能為主,VIP、SP和一氧化氮(NO)可能髮揮神經調質作用.
목적 관찰인정체신경-내장신경반사호건립후대서방광화분신경절(MPG)지간적신경통로급신경체질성질.방법 8지성년wistar대서재체건립인공체신경-내장신경반사호.장랄근과양화물매(HRP)주사지실험대서적방광벽기층,역행신경추종,통과면역조직화학혹조직화학방법현시HRP양성표기세포중적담감이선전이매(ChAT)、락안산간화매(TH)、혈관활성장태(VIP)、P물질(SP)화환원형보매Ⅱ(NADPH,NOS표지물).결과 지배방광적신경원다위우MPG주체부,정원형혹타원형,무명현돌기,다수위ChAT(40.1%,112/279)화TH(20.4%,50/245)면역양성,소수위VIP(10.7%,25/233)、NOS(6.5%,15/230)、SP(3.3%,8/242)반응양성.결론 인공체신경-내장신경반사호건립후MPG중지배방광적신경원이담감능화신상선소능위주,VIP、SP화일양화담(NO)가능발휘신경조질작용.
Objective Retrograde-tracing and immunohistochemical techniques were used in combination to investigate the types of putative transmitters in pelvic ganglia neurons projecting to the bladder of rats with the artificial somatic-autonomic reflex arc. Methods The artificial somatic-autonomic reflex arc was established in 8 rats by end-to-end microanastomosis of the proximal stump of the left L4 ventral root to the distal stump of L6 ventral root. The major pelvic ganglia (MPG) neurons innervating the urinary bladder were labeled by retrograde axonal tracing methods with the use of horseradish peroxidase (HRP) injected into the bladder wall and identified with TMB-HRP reaction. Then immunohistochemical (ChAT, TH, VIP, SP) and histochemical methods (NADPH-diaphorase activity and nitric oxide synthase immunoreac-tivity) were used to identify the neurotransmitters of HRP-labeled neurons projecting to the bladder. Results HRP-labeled neurons with spherical or oval shape mainly distributed in the MPG. The HRP-labeled neurons were positive for ChAT (112/279, 40. 1%), TH (50/245,20.4%), VIP(25/233, 10.7%), NOS ( 15/230, 6. 5% ) or SP (8/242, 3. 3% ) respectively. Conclusion These results suggest that ace-tylcholine and adrenaline are the predominant neurotransmitters of postganglionic neurons innervating bladder in rats with the artificial somatic-autonomic reflex arc. VIP, NO and SP may be as the neurotransmitters or neuromodulators to participate the procedure of urinary storage and voiding.
