CAJ | 학술논문

目的测定3株格林.巴利综合征(Guillain-Barre syndrome,GBS)相关宅肠弯曲菌的gale基因序列,并同GenBank中的空肠弯曲菌菌株相应序列进行比较,了解致GBS的序列特征并分析其遗传进化关系.方法选取分离自GBS患者粪便并经动物模型证实为致GBS的3株AMAN型空肠弯曲菌菌株进行培养并提取基因组DNA测序.将基因测序结果通过与NCTC11168菌株进行对照比较寻找galE基因突变位点并对gaZE基因片段进行遗传距离计算.结果 3株致GBS空肠弯曲菌菌株的galE基因均由987个碱基构成.与NCTC11168的galE基因序列相比,此3株空肠弯曲菌菌株gale基因核苷酸序列有4个相同碱基突变并导致了4个对应的相同氨基酸突变.遗传距离计算,zhanxing株与qiaoyuntao株距离为1.5%,zhanxing株与lulei株距离为1.6%,qiaoyuntao株与lulei株距离为0.5%.结论 GBS相关空肠弯曲菌中galE基因核苷酸序列的确存在相同变异且发生变异概率较非GBS相关空肠弯曲菌明显增大,遗传距离反映了此3株致GBS的空肠弯曲菌具有一定的区域特征.
목적 측정3주격림.파리종합정(Guillain-Barre syndrome,GBS)상관택장만곡균적gale기인서렬,병동GenBank중적공장만곡균균주상응서렬진행비교,료해치GBS적서렬특정병분석기유전진화관계.방법 선취분리자GBS환자분편병경동물모형증실위치GBS적3주AMAN형공장만곡균균주진행배양병제취기인조DNA측서.장기인측서결과통과여NCTC11168균주진행대조비교심조galE기인돌변위점병대gaZE기인편단진행유전거리계산.결과 3주치GBS공장만곡균균주적galE기인균유987개감기구성.여NCTC11168적galE기인서렬상비,차3주공장만곡균균주gale기인핵감산서렬유4개상동감기돌변병도치료4개대응적상동안기산돌변.유전거리계산,zhanxing주여qiaoyuntao주거리위1.5%,zhanxing주여lulei주거리위1.6%,qiaoyuntao주여lulei주거리위0.5%.결론 GBS상관공장만곡균중galE기인핵감산서렬적학존재상동변이차발생변이개솔교비GBS상관공장만곡균명현증대,유전거리반영료차3주치GBS적공장만곡균구유일정적구역특정.
Objective To investigate the Camtrylobacterjejuni's risk factors which were associated with the development of Guillain-Barre syndrome( GBS), the galE gene of C. jejuni strains were sequenced and the sequencing results were compared with other C. jejuni strains. Methods Selecting three GBS-asso-ciated C.jejuni strains isolated from stools of GBS patients who had been diagnosed as AMAN pattern by clin-ical and electrophysiological test from Hebei province, China. After sequencing galE gene, the results were spliced and assembled into a complete sequence by the terminals overlapped each other. The sequences of galE gene were compared with the corresponding sequences in GenBank to find the mutation and constructed the phylogenetic tree. Results The variation frequency of galE sequences of GBS-associated C. jejuni were higher than that of non-GBS-associated C. jejuni. The phylogenetic analysis demonstrated that each of the three C. jejuni strains was separately genetically closed to three strains which sequences have published in GenBank. The alignment with the related sequence of NCTC11168 shows that there are 4 same mutations in the galE gene of the three C. jejuni strains. The phylogenic tree reflected the regional feature of C. jejuni. Conclusion The probability of sequence variation of galE of GBS-associated C.jejuni is significantly higher than non-GBS-associated C. jejuni strains, the relation between the variation and GBS-pathogenesis remains to be further confirmed. The mutations found in the three C. jejuni strains established the foundation for ex-ploring the biologically characteristic of GBS-associated C. jejuni strains.