浙江大学学报(英文版)
浙江大學學報(英文版)
절강대학학보(영문판)
JOURNAL OF ZHEJIANG UNIVERSITY SCIENCE
2004年
8期
936-940
,共5页
江兵兵%高长有%胡玲%沈家骢
江兵兵%高長有%鬍玲%瀋傢驄
강병병%고장유%호령%침가총
Co-precipitation%Nanoparticles%Bone morphogenetic proteins%Biopolymers
A modified complex coacervation-co-precipitation method was used to prepare bone morphogenetic protein(BMP)-loaded nanospheres. Three natural polymers were used as packing materials to obtain nanoscale delivery device for BMP,in the presence of phosphatidylcholine functioning as stabilizer. Positively charged polysaccharide, N,N-diethylaminoethyl dex-tran (DEAE-dextran) tended to form stable, uniform and smaller size particles carrying BMP. Negatively charged bovine serumalbumin (BSA) induced precipitation of the produced BMP particles due to its weak interaction with BMP molecules, although itproduced nanosized BMP spheres. While collagen, a weakly positively charged protein shaped larger particles due to the stronginteraction among themselves. A mechanism of co-precipitation process was also deduced to depict the formation of stablenanospheres.
