CAJ | 학술논문

三种血红素类蛋白质--细胞色素c、肌红蛋白、血红蛋白,被固定在纳米氧化铝-金胶自组装体系修饰玻碳电极表面,紫外光谱实验结果表明固定在纳米氧化铝-金胶表面的蛋白质保持其原始的二级结构不变.用电化学阻抗光谱和循环伏安技术表征了界面的组装过程及其电化学性质,结果表明纳米氧化铝-金胶模板不仅为蛋白质固定提供了良好的环境,而且加快了蛋白质分子与电极之间的电子转移.讨论了扫描速度对细胞色素c电化学行为的影响及其对过氧化氢的电催化还原等性质.
삼충혈홍소류단백질--세포색소c、기홍단백、혈홍단백,피고정재납미양화려-금효자조장체계수식파탄전겁표면,자외광보실험결과표명고정재납미양화려-금효표면적단백질보지기원시적이급결구불변.용전화학조항광보화순배복안기술표정료계면적조장과정급기전화학성질,결과표명납미양화려-금효모판불부위단백질고정제공료량호적배경,이차가쾌료단백질분자여전겁지간적전자전이.토론료소묘속도대세포색소c전화학행위적영향급기대과양화경적전최화환원등성질.
Three heme proteins, cytochrome c, myoglobin and hemoglobin were adsorbed on the nanoporous alumina (AAO) templates-gold nanoparticles (GNs) modified glassy carbon electrodes (GCE). UV-vis spectroscopy indicated that the proteins immobilized on AAO-GNs films retained near-native secondary structures. The fabricated procedures and electrochemical behaviors of proteins on such an interface were characterized with electrochemical impedance spectra and cyclic voltammetric techniques. It was demonstrated that the AAO-GNs films not only could offer a friendly environment to immobilize the protein molecules, but also enhance the electron transfer ability between protein molecules and the underlying electrodes. The effects of scan rate on the electrochemical behaviors of cytochrome c and the electrocatalytic response to the reduction of H2O2 were discussed in detail.