CAJ | 학술논문

背景与目的:肿瘤高选择性摄入光敏剂的原因,即靶向原理至今仍不清楚.本研究探讨肿瘤细胞对光敏剂的亲和力.方法:应用紫外分光光度仪测定各种细胞培养液的吸收光谱:应用荧光分光光度仪对光敏剂photofrin-Ⅱ进行荧光光谱分析.同时测定人永生化食管上皮细胞系SHEE及其癌变细胞系SHEEC在相同浓度和时间点对photofrin-Ⅱ的吸收和排泄.结果:photofrin-Ⅱ的最大荧光激发波长为(395.0±0.5)nm,最大荧光发射波长为(634.1±0.5)nm;光敏剂photofrin-Ⅱ所需要的630 nm激发波长的光,可完全通过各种细胞培养液.相同浓度和时间下,SHEE细胞和SHEEC细胞对photofrin-Ⅱ的吸收和排泄差异无统计学意义(P>0.05);SHEE细胞和SHEEC细胞对光敏剂photofrin-Ⅱ的吸收量均随其浓度的增加而增加,30 μg/mL后进入平台期;SHEE细胞和SHEEC细胞对光敏剂photofrin-Ⅱ的吸收量随着孵育时间的延长而增高,150 min后进入平台期;photofrin-Ⅱ在SHEE细胞和SHEEC细胞内可保持较高水平15～30 min,然后迅速排出.结论:光敏剂在肿瘤组织中浓集现象可能与肿瘤细胞亲和力无关系.
배경여목적:종류고선택성섭입광민제적원인,즉파향원리지금잉불청초.본연구탐토종류세포대광민제적친화력.방법:응용자외분광광도의측정각충세포배양액적흡수광보:응용형광분광광도의대광민제photofrin-Ⅱ진행형광광보분석.동시측정인영생화식관상피세포계SHEE급기암변세포계SHEEC재상동농도화시간점대photofrin-Ⅱ적흡수화배설.결과:photofrin-Ⅱ적최대형광격발파장위(395.0±0.5)nm,최대형광발사파장위(634.1±0.5)nm;광민제photofrin-Ⅱ소수요적630 nm격발파장적광,가완전통과각충세포배양액.상동농도화시간하,SHEE세포화SHEEC세포대photofrin-Ⅱ적흡수화배설차이무통계학의의(P>0.05);SHEE세포화SHEEC세포대광민제photofrin-Ⅱ적흡수량균수기농도적증가이증가,30 μg/mL후진입평태기;SHEE세포화SHEEC세포대광민제photofrin-Ⅱ적흡수량수착부육시간적연장이증고,150 min후진입평태기;photofrin-Ⅱ재SHEE세포화SHEEC세포내가보지교고수평15～30 min,연후신속배출.결론:광민제재종류조직중농집현상가능여종류세포친화력무관계.
Background and Objective:The mechanism of tumor tissues selectively uptaking the photosensitizer in photodynamic therapy(PDT)is still unclear.This study was to investigate the affinity of tumor cells to the photosensitizer photofrin-Ⅱ. Methods: Ultraviolet spectrophotometer was applied to measure the absorption spectra of various cell culture media.The fluorescence spectrum of photofrin-Ⅱ was determined by spectrofluorometer.The absorption and eIimination condition of photofrin-Ⅱ were detected in immortalized human esophageal epithelial cell line SHEE and its malignant transformation cell line SHEEC.Results:The maximum excitation wavelength of fluorescence for photofrin-Ⅱ was(395.0±0.5) nm, and the maximum emission wavelength of that was (634.1±0.5) nm.The laser at the wavelength of 630 nm used in this experiment could permeate various types of cell culture media.There was no significant difference in the absorption and elimination of photofrin-Ⅱ between SHEE and SHEEC at the same concentration and time.The absorption of photofrin-Ⅱ in SHEE and SHEEC increased with the increase in photofrin-Ⅱ concentration and duration. and reached the platform at the concentration of 30 μg/mL and a time point of 150 min.respectively.The photofrin-Ⅱ contents of SHEE and SHEEC showed a slight change after 15-30 min, and diminished rapidly after 30 min.Conclusion:High photosensitizer concentration in tumor tissues may be not correlated with the affinity of tumor cells.