临床与实验病理学杂志
臨床與實驗病理學雜誌
림상여실험병이학잡지
CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2009年
6期
624-627
,共4页
冀学宁%范学军%李梅%吕申%王若雨
冀學寧%範學軍%李梅%呂申%王若雨
기학저%범학군%리매%려신%왕약우
肝肿瘤%NF-κB%肿瘤转移
肝腫瘤%NF-κB%腫瘤轉移
간종류%NF-κB%종류전이
liver neoplasms%NF-κB%neoplams metastasis
目的 探讨NF-κB基因表达在小鼠肝癌淋巴道转移细胞系中的表达及意义.方法 采用RQ-PCR方法检测NF-κB基因在肝癌淋巴道低转移潜能的小鼠肝癌细胞系Hca-P和高转移潜能的小鼠肝癌细胞系Hca-F中的表达水平.结果 NF-κB基因在Hca-P和Hca-F细胞系基因表达分别为(1.41±0.48)×10~(-3)、(2.95±0.22)×10~(-3),Hca-P和 Hca-F之间,NF-κB基因表达差异有统计学意义 (P<0.01),其随转移潜能的增高,表达水平增加.结论 NF-κB基因表达水平增高与肝癌淋巴道侵袭转移相关.
目的 探討NF-κB基因錶達在小鼠肝癌淋巴道轉移細胞繫中的錶達及意義.方法 採用RQ-PCR方法檢測NF-κB基因在肝癌淋巴道低轉移潛能的小鼠肝癌細胞繫Hca-P和高轉移潛能的小鼠肝癌細胞繫Hca-F中的錶達水平.結果 NF-κB基因在Hca-P和Hca-F細胞繫基因錶達分彆為(1.41±0.48)×10~(-3)、(2.95±0.22)×10~(-3),Hca-P和 Hca-F之間,NF-κB基因錶達差異有統計學意義 (P<0.01),其隨轉移潛能的增高,錶達水平增加.結論 NF-κB基因錶達水平增高與肝癌淋巴道侵襲轉移相關.
목적 탐토NF-κB기인표체재소서간암림파도전이세포계중적표체급의의.방법 채용RQ-PCR방법검측NF-κB기인재간암림파도저전이잠능적소서간암세포계Hca-P화고전이잠능적소서간암세포계Hca-F중적표체수평.결과 NF-κB기인재Hca-P화Hca-F세포계기인표체분별위(1.41±0.48)×10~(-3)、(2.95±0.22)×10~(-3),Hca-P화 Hca-F지간,NF-κB기인표체차이유통계학의의 (P<0.01),기수전이잠능적증고,표체수평증가.결론 NF-κB기인표체수평증고여간암림파도침습전이상관.
Purpose To study NF-κB gene expression level in mouse hepatocellular carcinoma cell lines with differently lymphatic metastasis potentials and to discuss its roles in lymphatic metastasis.Methods Using real-time quantitative PCR, NF-κB gene expression level was detected in mouse hepatocellular carcinoma cell lines, including Hca-P with low lymphatic metastasis potential and Hca-F with high lymphatic metastasis potential.Results NF-κB mRNA expression in Hca-P and Hca-F cell lines were (1.41±0.48)×10~(-3),and (2.95±0.22)×10~(-3) (P<0.01),respectively.NF-κB mRNA expression levels were increased with metastasis potential.Conclusion NF-κB gene may play an important role in lymphatic metastasis of hepatocellular carcinoma.
