中成药
中成藥
중성약
CHINESE TRADITIONAL PATENT MEDICINE
2010年
3期
374-378
,共5页
张舒%沈明勤%丁永芳%王小宁
張舒%瀋明勤%丁永芳%王小寧
장서%침명근%정영방%왕소저
复方肠泰%人结肠癌细胞SW480%细胞周期%细胞增殖%凋亡
複方腸泰%人結腸癌細胞SW480%細胞週期%細胞增殖%凋亡
복방장태%인결장암세포SW480%세포주기%세포증식%조망
Compound Changtai Granule%human colon carcinoma cell line SW480%cell Cycle%cell proliferation%apoptosis
目的:考察复方肠泰(人参、葸苡仁、莪术等)对人结肠癌细胞SW480细胞生长的抑制作用及其对细胞增殖周期和凋亡的影响.方法:采用噻唑蓝比色法测定不同浓度复方肠泰在不同作用时间内对在体外培养SW480细胞增殖的影响,同时应用流式细胞术检测细胞增殖周期及凋亡率的变化.结果:复方肠泰可在G_0/G_1期以及G_2/M期阻滞人结肠癌细胞SW480的增殖,使S期细胞比率降低.结论:复方肠泰在3.51～7.81 mg/mL范围内呈现明显的量效关系.
目的:攷察複方腸泰(人參、葸苡仁、莪術等)對人結腸癌細胞SW480細胞生長的抑製作用及其對細胞增殖週期和凋亡的影響.方法:採用噻唑藍比色法測定不同濃度複方腸泰在不同作用時間內對在體外培養SW480細胞增殖的影響,同時應用流式細胞術檢測細胞增殖週期及凋亡率的變化.結果:複方腸泰可在G_0/G_1期以及G_2/M期阻滯人結腸癌細胞SW480的增殖,使S期細胞比率降低.結論:複方腸泰在3.51～7.81 mg/mL範圍內呈現明顯的量效關繫.
목적:고찰복방장태(인삼、사이인、아술등)대인결장암세포SW480세포생장적억제작용급기대세포증식주기화조망적영향.방법:채용새서람비색법측정불동농도복방장태재불동작용시간내대재체외배양SW480세포증식적영향,동시응용류식세포술검측세포증식주기급조망솔적변화.결과:복방장태가재G_0/G_1기이급G_2/M기조체인결장암세포SW480적증식,사S기세포비솔강저.결론:복방장태재3.51～7.81 mg/mL범위내정현명현적량효관계.
AIM:To check the antiproliferative and apoptosis-inducing effect of Compound Changtai Granule(Radix et Rhizoma ginseng,Semen coicis,Rhizoma curcumae,etc)(CCG)on human colon carcinoma cell line(SW480).METHODS:Methy thiazolyl tetrazolium chromatomerry was used to observe the influence of several concentration of CCG on SW480 cell proliferation cultured in vitro at the various times,and flow cytometry was applied to examining the change in SW480 cell proliferation cycle and apoptosis rate.RESULTS:CCG could successfully prevent SW480 cell from entering G_0/G_1 phase and G_2/M phase,decrease cell numbers in S phase at the same time.CONCLUSION:CCG has an obvious dose-response relationship in the range of 3.51 to 7.81 mg/mL.
