CAJ | 학술논문

为分析2型糖尿病患者神经-内分泌-免疫网络紊乱情况及其临床意义,选择174例经临床诊断明确的2型糖尿病患者,用放射免疫法和流式细胞技术检测外周血中CD4+、CD8+、NK细胞数量、CD4+/CD8+比值及IL-1β、IL-6、TNF-α、ACTH、CORT、DA、NE水平,选择30例健康志愿者为正常对照组进行相同检测.结果显示,与正常对照相比,2型糖尿病患者CD4+、CD8+及NK细胞数量下降(P＜0.01),CD8+及CD4+/CD8+比值升高(P＜0.01),IL-1β、IL-6和TNF-α均有升高(P＜0.01),ACTH及CORT下降(P＜0.01),DA和NE升高(P＜0.01).研究表明,2型糖尿病患者神经-内分泌-免疫网络的紊乱中,其体液免疫增强、细胞免疫减弱,交感神经兴奋及应激性内分泌功能减弱为主要表现.对于2型糖尿病的治疗,除采用现有手段,应加强机体对胰岛素抵抗调控平衡态的修复与重建,重视神经-内分泌-免疫系统标志的检测及相应的新治疗研究.
위분석2형당뇨병환자신경-내분비-면역망락문란정황급기림상의의,선택174례경림상진단명학적2형당뇨병환자,용방사면역법화류식세포기술검측외주혈중CD4+、CD8+、NK세포수량、CD4+/CD8+비치급IL-1β、IL-6、TNF-α、ACTH、CORT、DA、NE수평,선택30례건강지원자위정상대조조진행상동검측.결과현시,여정상대조상비,2형당뇨병환자CD4+、CD8+급NK세포수량하강(P＜0.01),CD8+급CD4+/CD8+비치승고(P＜0.01),IL-1β、IL-6화TNF-α균유승고(P＜0.01),ACTH급CORT하강(P＜0.01),DA화NE승고(P＜0.01).연구표명,2형당뇨병환자신경-내분비-면역망락적문란중,기체액면역증강、세포면역감약,교감신경흥강급응격성내분비공능감약위주요표현.대우2형당뇨병적치료,제채용현유수단,응가강궤체대이도소저항조공평형태적수복여중건,중시신경-내분비-면역계통표지적검측급상응적신치료연구.
To investigate the disorder of neuro-endocrine-immune network in patients with type-2 diabetes milletus and its clinical significance,174 patients with clinical proven type-2 diabetes mellitus were enrolled in the study.Radio-immune technology and flow cytometer technology were used to detect the blood levels of CD4+,CD8+,NK and CD4+/CD8+,and cytokines including IL-1β,IL-6,TNF α,ACTH,CORT and DA as well as NE.Analyses were performed on the basis of gender with 33 normal people served as the control group.A significant decrease of CD4+,CD8+,NK lymphocytes count,and a significant increase of CD4+/CD8+ were found in type-2 diabetes milletus patients compared with the control group (P＜0.01).Low IL-1β,IL-6 and TNF-α levels were found in type-2 diabetes milletus groups (P＜0.01).The serum ACTH,CORT levels were decreased in type-2 diabetes milletus patients (P＜0.01);both serum DA and NE levels were increased in type-2 diabetes milletus groups compared with the normal group (P＜0.01).The disturbance of neuro endocrine-immune network in patients with type-2 diabetes milletus is characterized by the humoral immunity activated and cellular immunity depressed;symphathetic nerve activated;the stress related endocrine depressed.It is important to strengthen and recover the balance of neuro-endocrine-immune network,for the treatment of type-2 diabetes mellitus,which is all important issue for the future research on diabetes.