国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2010年
6期
540-541,543
,共3页
抗体,抗核%抗原%自身免疫疾病
抗體,抗覈%抗原%自身免疫疾病
항체,항핵%항원%자신면역질병
antibodies,antinuclear%antigens%autoimmune diseases
目的 通过对抗核抗体、可提取性核抗原(ENA)及抗核抗体谱3检测方法 的分析比较,探讨有益于临床诊断的检测组合.方法 采用人喉癌上皮细胞及猴肝作为基质的间接免疫荧光法检测抗核抗体;应用欧蒙斑点法检测可提取性核抗原中的如下抗原:nRNP/Sm、Sm、SSA、SSB、Scl-70、Jo-1;以绿蝇短膜虫为基质,应用间接免疫荧光法检测抗双链DNA抗体;通过欧蒙印迹法检测15种自身抗体.结果 将3种稀释度(1:10、1:80、1:100)抗核抗体检测的阳性率进行比较,1:10及1:80稀释的血清抗核抗体阳性率差异有统计学意义(P<0.01),其余2种比较差异无统计学意义;在核点型、胞浆颗粒型及均质型等多种核型的检测中抗核抗体谱3的阳性检出率高于ENA谱;在抗双链DNA抗体的检测中间接免疫荧光法的阳性检出率略高于欧蒙印迹法.结论 在应用间接免疫荧光法的抗核抗体检测中,使用1:80的稀释度更有利于提高检出率,而且涵盖15种抗原的抗核抗体谱3也为临床诊断提供了有利的依据.
目的 通過對抗覈抗體、可提取性覈抗原(ENA)及抗覈抗體譜3檢測方法 的分析比較,探討有益于臨床診斷的檢測組閤.方法 採用人喉癌上皮細胞及猴肝作為基質的間接免疫熒光法檢測抗覈抗體;應用歐矇斑點法檢測可提取性覈抗原中的如下抗原:nRNP/Sm、Sm、SSA、SSB、Scl-70、Jo-1;以綠蠅短膜蟲為基質,應用間接免疫熒光法檢測抗雙鏈DNA抗體;通過歐矇印跡法檢測15種自身抗體.結果 將3種稀釋度(1:10、1:80、1:100)抗覈抗體檢測的暘性率進行比較,1:10及1:80稀釋的血清抗覈抗體暘性率差異有統計學意義(P<0.01),其餘2種比較差異無統計學意義;在覈點型、胞漿顆粒型及均質型等多種覈型的檢測中抗覈抗體譜3的暘性檢齣率高于ENA譜;在抗雙鏈DNA抗體的檢測中間接免疫熒光法的暘性檢齣率略高于歐矇印跡法.結論 在應用間接免疫熒光法的抗覈抗體檢測中,使用1:80的稀釋度更有利于提高檢齣率,而且涵蓋15種抗原的抗覈抗體譜3也為臨床診斷提供瞭有利的依據.
목적 통과대항핵항체、가제취성핵항원(ENA)급항핵항체보3검측방법 적분석비교,탐토유익우림상진단적검측조합.방법 채용인후암상피세포급후간작위기질적간접면역형광법검측항핵항체;응용구몽반점법검측가제취성핵항원중적여하항원:nRNP/Sm、Sm、SSA、SSB、Scl-70、Jo-1;이록승단막충위기질,응용간접면역형광법검측항쌍련DNA항체;통과구몽인적법검측15충자신항체.결과 장3충희석도(1:10、1:80、1:100)항핵항체검측적양성솔진행비교,1:10급1:80희석적혈청항핵항체양성솔차이유통계학의의(P<0.01),기여2충비교차이무통계학의의;재핵점형、포장과립형급균질형등다충핵형적검측중항핵항체보3적양성검출솔고우ENA보;재항쌍련DNA항체적검측중간접면역형광법적양성검출솔략고우구몽인적법.결론 재응용간접면역형광법적항핵항체검측중,사용1:80적희석도경유리우제고검출솔,이차함개15충항원적항핵항체보3야위림상진단제공료유리적의거.
Objective To study more significant detective combination by comparing three methods of antinuclear antibody,extractable nuclear antigen and antinuclear antibodies 3.Methods Three methods were adopted as follows:detecting antinuclear antibody with HEP-2 and prime hepar as antigen media,determining extractable nuclear antigen by EUROASSAY including nRNP/Sm、Sm、SSA、SSB、Scl-70 and Jo-1,using crithidia iuciliae as antigen,to detect ds-DNA antibody with indirect immunofluorescence assay,and to study 15 autoantibodies by EUROLINE.Results To compare positive rates of three dilution(1:10,1:80,1:100)in ANA detection,there existed significant difference(P<0.01)between two dilution of 1:10 and 1:80 only,.In the detection of multiple nuclear types(cytoplasmic pattern,centromere pattern and homegeneous pattern etc),the positive rate of antinucleatr antibodies 3 was higer than that of ENA.IIF was prior to the EUROLINE in the detection of ds-DNA.Conclusion During detecting ANA with IIF,the dilution of 1:80 has advantages in raising positive rate,and ANA3 of 15 antigens provides facilities for clinical diagnosis.
