国际输血及血液学杂志
國際輸血及血液學雜誌
국제수혈급혈액학잡지
INTERNATIONAL JOURNAL OF BLOOD TRANSFUSION AND HEMATOLOGY
2011年
1期
14-16
,共3页
RhD-%血清学分型%序列特异性引物聚合酶链反应%基因分型%遗传背景
RhD-%血清學分型%序列特異性引物聚閤酶鏈反應%基因分型%遺傳揹景
RhD-%혈청학분형%서렬특이성인물취합매련반응%기인분형%유전배경
RhD-%Serological typing%PCR-SSP%Genotyping%Genetic background
目的 比较和评价血清学和序列特异性引物聚合酶链反应(PCR-SSP)基因分型技术对RhD-先证者及家系成员Rh血型分型和遗传背景分析的效果.方法 对RhD-先证者及其家系成员,采用血清学分型技术检测其RhD、C、c、E、e抗原;采用PCR-SSP基因分型技术检测其RHD、RHCE基因.结果 先证者家系成员Rh表型正常,PCR结果与血清学表型相符;先证者RHD基因特异性条带全部检出,未检出RHCE基因特异性序列;家系成员RHD、RHCE基因未见异常.结论 血清学方法和PCR-SSP基因分型技术在RhD-诊断中具有互补性,结合两种技术有助于分析其遗传背景.
目的 比較和評價血清學和序列特異性引物聚閤酶鏈反應(PCR-SSP)基因分型技術對RhD-先證者及傢繫成員Rh血型分型和遺傳揹景分析的效果.方法 對RhD-先證者及其傢繫成員,採用血清學分型技術檢測其RhD、C、c、E、e抗原;採用PCR-SSP基因分型技術檢測其RHD、RHCE基因.結果 先證者傢繫成員Rh錶型正常,PCR結果與血清學錶型相符;先證者RHD基因特異性條帶全部檢齣,未檢齣RHCE基因特異性序列;傢繫成員RHD、RHCE基因未見異常.結論 血清學方法和PCR-SSP基因分型技術在RhD-診斷中具有互補性,結閤兩種技術有助于分析其遺傳揹景.
목적 비교화평개혈청학화서렬특이성인물취합매련반응(PCR-SSP)기인분형기술대RhD-선증자급가계성원Rh혈형분형화유전배경분석적효과.방법 대RhD-선증자급기가계성원,채용혈청학분형기술검측기RhD、C、c、E、e항원;채용PCR-SSP기인분형기술검측기RHD、RHCE기인.결과 선증자가계성원Rh표형정상,PCR결과여혈청학표형상부;선증자RHD기인특이성조대전부검출,미검출RHCE기인특이성서렬;가계성원RHD、RHCE기인미견이상.결론 혈청학방법화PCR-SSP기인분형기술재RhD-진단중구유호보성,결합량충기술유조우분석기유전배경.
Objective To evaluate the effects of serological typing and PCR-SSP genotyping which were used for Rh blood typing and genetic basis of the Rh D- index case and her family members. Methods Rh D,C, c, E, e antigens were detected by serological typing. The characteristic sequence fragments of RHD and RHCE were amplified by PCR-SSP with a commercial reagent. Results Rh phenotypes of the proband' s family members were normal. The genotypes of PCR were consistent with their serological phenotypes. The full specific RHD bands of the proband were found. No specific RHCE bands were detected. The full specific bands of RHD and RHCE of her family members were normal. Conclusion Serological typing and PCR-SSP genotyping were complementary to RhD- diagnosis. Combination of the two technologies can help to analyze the genetic background of RhD-.
