CAJ | 학술논문

目的探讨肥大细胞在先天性巨结肠小肠结肠炎发病机制的作用,为临床治疗提供依据.方法 2004年5月～2006年5月在上海交通大学医学院附属新华医院行先天性巨结肠根治术中切除的狭窄段、移行段和扩张段肠管黏膜层标本,共30例.男23例,女7例,年龄1个月～7岁,平均1.2岁.短段型5例,普通型18例,长段型6例,全结肠型1例.根据术前有无小肠结肠炎临床症状将患儿分为巨结肠肠炎组(n=12)和非肠炎组(n=18).肥大细胞采用甲苯胺蓝染色和免疫组织化学染色(chymase)染色.结果巨结肠肠炎组狭窄段、移行段和扩张段肥大细胞在黏膜,黏膜下及扩张的血管周围聚集,脱颗粒现象明显.非肠炎组结肠组织中肥大细胞在黏膜下,黏膜明显减少,脱颗粒现象轻微.结论肥大细胞在肠炎的发生中起一定的作用,采用肥大细胞抑制剂是一种新的临床治疗先天性巨结肠小肠结肠炎途径.
목적 탐토비대세포재선천성거결장소장결장염발병궤제적작용,위림상치료제공의거.방법 2004년5월～2006년5월재상해교통대학의학원부속신화의원행선천성거결장근치술중절제적협착단、이행단화확장단장관점막층표본,공30례.남23례,녀7례,년령1개월～7세,평균1.2세.단단형5례,보통형18례,장단형6례,전결장형1례.근거술전유무소장결장염림상증상장환인분위거결장장염조(n=12)화비장염조(n=18).비대세포채용갑분알람염색화면역조직화학염색(chymase)염색.결과 거결장장염조협착단、이행단화확장단비대세포재점막,점막하급확장적혈관주위취집,탈과립현상명현.비장염조결장조직중비대세포재점막하,점막명현감소,탈과립현상경미.결론 비대세포재장염적발생중기일정적작용,채용비대세포억제제시일충신적림상치료선천성거결장소장결장염도경.
Objective To assess the role of mast cell and Hirschsprung's disease(HD)associated enterocolitis.Methods We studied 30 patients(23 boys and 7 girls)with HD admitted into our hospital between May 2004 and May 2006.There were 5 short-segment,18 common type,6 long-segment type and 1 total colon type HD. The patients were divided into enterocolitis group(n=12)and non-enterocolitis group(n=18).The mast cells were irnmunostained with toluidine blue and chymase.Results The dilated vessels and the mast cells with degranulation were localized in mucosal and sub-mucosal layers in the enterocolitis group.In the non-enterocolitis group,the mast cells were mainly in submucosallayer and to less extent in the mucosal layer with minimal degranulation.Conclusions These results suggest that mast cells may be involved in the pathogenesis of Hischsprung's diseaseassociated enterocolitis.