CAJ | 학술논문

目的探讨含锶羟基磷灰石(Sr-HA)对骨髓基质干细胞(BMSCs)成骨分化的影响.方法从2只SD雄性大鼠双侧股骨、胫骨提取BMSCs.用含锶量分别为摩尔百分比为10%Sr-HA(A组)、5%Sr-HA(B组)、1% Sr-HA(C组)及不含锶的HA(D组)生物陶瓷粉末制备浸提液培养大鼠BMSCs.在培养的1、3、5、7、10 d检测各组BMSCs的增殖情况,于诱导培养后6、9、12及15 d检测各组BMSCs的碱性磷酸酶(ALP)活性,于诱导培养后3、6、9及12 d检测各组BMSCs的核心结合因子α1(Cbfα1)基因表达.结果 A、B、C、D组细胞培养1、3、5、7、10 d四甲基偶氮唑盐(MTT)检测结果显示:各组间吸光度值差异均无统计学意义(P＞0.05),但同一组内不同时间点的吸光度值差异均有统计学意义(P＜0.05).细胞培养6 d时A、B、C、D组的ALP活性OD值分别为(0.061±0.005)、(0.058±0.003)、(0.056±0.005)、(0.055±0.005),从6 d起A组和B组ALP活性均显著高于C、D组,差异有统计学意义(P＜0.05).细胞培养9和12 d时各组Cbfα1 mRNA相对表达量两两比较差异均有统计学意义(P＜0.05).结论 HA中含锶量对BMSCs增殖无明显影响,但能促进BMSCs的成骨分化.
목적 탐토함송간기린회석(Sr-HA)대골수기질간세포(BMSCs)성골분화적영향.방법 종2지SD웅성대서쌍측고골、경골제취BMSCs.용함송량분별위마이백분비위10%Sr-HA(A조)、5%Sr-HA(B조)、1% Sr-HA(C조)급불함송적HA(D조)생물도자분말제비침제액배양대서BMSCs.재배양적1、3、5、7、10 d검측각조BMSCs적증식정황,우유도배양후6、9、12급15 d검측각조BMSCs적감성린산매(ALP)활성,우유도배양후3、6、9급12 d검측각조BMSCs적핵심결합인자α1(Cbfα1)기인표체.결과 A、B、C、D조세포배양1、3、5、7、10 d사갑기우담서염(MTT)검측결과현시:각조간흡광도치차이균무통계학의의(P＞0.05),단동일조내불동시간점적흡광도치차이균유통계학의의(P＜0.05).세포배양6 d시A、B、C、D조적ALP활성OD치분별위(0.061±0.005)、(0.058±0.003)、(0.056±0.005)、(0.055±0.005),종6 d기A조화B조ALP활성균현저고우C、D조,차이유통계학의의(P＜0.05).세포배양9화12 d시각조Cbfα1 mRNA상대표체량량량비교차이균유통계학의의(P＜0.05).결론 HA중함송량대BMSCs증식무명현영향,단능촉진BMSCs적성골분화.
Objective To determine if strontium-containing hydroxyapatite (Sr-HA) will favor osteoblastic differentiation of bone masenchymal stromal cells (BMSCs). Methods Rat BMSCs were cultured with culture media containing ions released from the strontium-substituted HA ceramics as they dissolved. We had 3 experimental groups and one blank control group. In group A, leaching liquor containing 10 mol% of Sr-HA was used;in group B, leaching liquor containing 5 mol% of Sr-HA was used;in group C,leaching liquor containing 1 mol% of Sr-HA was used;in group D, leaching liquor containing no Sr-HA was used. MTT test, alkaline phosphatase (ALP) activity and osteoblast transcription factor gene (Cbfα1) expression examinations were conducted at different time-points. Results MTT test showed that there were no significant differences in absorbance between the 4 groups after cell culture for 1, 3, 5, 7, 10 days( P ＞ 0. 05),but there were significant differences in absorbance between different time-points within the same group ( P ＜0.05). The ALP activity in groups A and B was significantly greater than in groups C and D ( P ＜ 0. 05) at 6, 9,12, 15 days. At 9 and 12 days, there were significant differences in Cbfα1 mRNA expression between the 4 groups ( P ＜ 0. 05). Compared with the blank control group, Sr-HA groups presented a significant enhancement with regard to ALP activity and Cbfα1 mRNA expression in a concentration-dependent manner. Conclusion Incorportion of strontium in HA ceramics may not promote proliferation of BMSCs, but may stimulate osteoblastic differentiation of BMSCs.