中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2008年
10期
944-946
,共3页
侯月梅%娜几娜%热依兰·艾沙
侯月梅%娜幾娜%熱依蘭·艾沙
후월매%나궤나%열의란·애사
微电极%心脏%心肌%细胞培养
微電極%心髒%心肌%細胞培養
미전겁%심장%심기%세포배양
Microelectrodes%Heart%Myocardium%Cell culture
目的 探讨微电极阵芯片技术(mieroelectrode arrays,MEA)在整体心脏、心肌组织片和培养心肌电活动的应用.方法 用MEA技术记录豚鼠整体心脏、SD大鼠心脏组织片和小鼠乳鼠心脏培养心肌的组织场电位变化规律.结果 (1)豚鼠整体心脏灌流30～90 min,记录到稳定的自主节律(120±90)次/min;心室和心房肌的场电位时程为(210±78)ms和(164±58)ms,房室传导时间为(320±150)ms.(2)SD大鼠心脏组织片兴奋性可以保持2 h,心室和心房组织的场电位时程为(115.80±11.61)ms和(83.71±6.48)ms.(3)小鼠乳鼠心脏原代培养自发搏动频率为(150±100)次/min,培养2～72 h,记录到同步的多位点场电位,并能随刺激频率同步显示场电位变化.结论 MEA技术是一个敏感、低噪音和长时间稳定的记录整体动物心脏、心脏靶点取样组织和培养心肌细胞的场电位、细胞间电活动传导和激动起源规律的研究工具.
目的 探討微電極陣芯片技術(mieroelectrode arrays,MEA)在整體心髒、心肌組織片和培養心肌電活動的應用.方法 用MEA技術記錄豚鼠整體心髒、SD大鼠心髒組織片和小鼠乳鼠心髒培養心肌的組織場電位變化規律.結果 (1)豚鼠整體心髒灌流30～90 min,記錄到穩定的自主節律(120±90)次/min;心室和心房肌的場電位時程為(210±78)ms和(164±58)ms,房室傳導時間為(320±150)ms.(2)SD大鼠心髒組織片興奮性可以保持2 h,心室和心房組織的場電位時程為(115.80±11.61)ms和(83.71±6.48)ms.(3)小鼠乳鼠心髒原代培養自髮搏動頻率為(150±100)次/min,培養2～72 h,記錄到同步的多位點場電位,併能隨刺激頻率同步顯示場電位變化.結論 MEA技術是一箇敏感、低譟音和長時間穩定的記錄整體動物心髒、心髒靶點取樣組織和培養心肌細胞的場電位、細胞間電活動傳導和激動起源規律的研究工具.
목적 탐토미전겁진심편기술(mieroelectrode arrays,MEA)재정체심장、심기조직편화배양심기전활동적응용.방법 용MEA기술기록돈서정체심장、SD대서심장조직편화소서유서심장배양심기적조직장전위변화규률.결과 (1)돈서정체심장관류30～90 min,기록도은정적자주절률(120±90)차/min;심실화심방기적장전위시정위(210±78)ms화(164±58)ms,방실전도시간위(320±150)ms.(2)SD대서심장조직편흥강성가이보지2 h,심실화심방조직적장전위시정위(115.80±11.61)ms화(83.71±6.48)ms.(3)소서유서심장원대배양자발박동빈솔위(150±100)차/min,배양2～72 h,기록도동보적다위점장전위,병능수자격빈솔동보현시장전위변화.결론 MEA기술시일개민감、저조음화장시간은정적기록정체동물심장、심장파점취양조직화배양심기세포적장전위、세포간전활동전도화격동기원규률적연구공구.
Objective To observe field potentials (FPs) and activation sequence at Langendorff perfused guinea pigs heart,SD rat cardiac tissue strips perfnsed by Tyrede's solution and cultured ventricular cardiomyocytes of suckling mice by microehtrode arrays (MEA) technique.Method FPs and activation sequence were recorded from perfnsed heart,cardiac tissue strips (5 mm×5 mm) and cultured ventricular cardiomyocytes by MEA.Results (1) FPs could be recorded in hearts perfused for 30 to 90 min with a heart rate 90-120 beats/mitt FP durations of beth ventricular and atrial tissue were (210±78) ms and (164±58) ms,respectively and atrial ventricular conduction time wag (320±150) ms.(2) The excitability of Tyrode's solution perfused tissue strips wag risible for more than 2 h,and FP durations of ventricular and atrial tissue strips were (115.80 ±11.61) ms and (83.71 ±6.48) ms,respectively.(3)Spontaneous beating frequency (150±100) beats/rain and FPs could be readily recorded in cardiomyocytes cultured between 2 to 72 hours.Conclusion MEAs is a sensitive,low noise and stable technique for observing local tissue action potential and activation sequence of whole heart,cardiac tissue strips and cultured cardiomyoeytes.
