中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2008年
6期
441-443
,共3页
姜文霞%宋伯根%汤如勇%方建萍
薑文霞%宋伯根%湯如勇%方建萍
강문하%송백근%탕여용%방건평
胆囊肿瘤%nm23%KAI1%免疫组织化学
膽囊腫瘤%nm23%KAI1%免疫組織化學
담낭종류%nm23%KAI1%면역조직화학
Gallbladder neoplasms%nm23%KAI1%Immunohistochemistry
目的 探讨肿瘤转移抑制基因nm23和KAI1在胆囊腺癌中的表达及其临床意义.方法 收集31例胆囊腺癌患者的病理石蜡标本和临床病理资料,将标本连续切片进行免疫组化染色,检测nm23、KAI1的表达.所有患者随访3年.结果 31例患者中,nm23阳性表达22例(71.0%),KAI1阳性表达19例(61.3%).早期与中晚期患者的nm23和KAI1阳性表达率差异均有统计学意义(P确切=0.0051,P确切=0.0084),且两基因阳性率与临床分期呈负相关(P趋势=0.0047,P趋势=0.0058).高、中、低分化胆囊腺癌的nm23和KAI1阳性表达率差异均有统计学意义(P确切=0.0328,P确切=0.0020),且两基因阳性率与组织学分级呈正相关(P趋势=0.0086,P趋势=0.0006).术后3年,存活(17例)与死亡者(14例)的nm23和KAI1阳性表达率差异均有统计学意义(P确切=0.0038,P确切=0.0001),存活者中两基因均表达阳性15例,而死亡者中两基因均表达阴性7例(P确切=0.0005).结论 nm23和KAI1蛋白表达与胆囊腺癌的临床分期呈负相关,与组织学分级呈正相关.nm23和KAI1蛋白高表达预示胆囊腺癌患者预后较好,并且两基因有协同作用.
目的 探討腫瘤轉移抑製基因nm23和KAI1在膽囊腺癌中的錶達及其臨床意義.方法 收集31例膽囊腺癌患者的病理石蠟標本和臨床病理資料,將標本連續切片進行免疫組化染色,檢測nm23、KAI1的錶達.所有患者隨訪3年.結果 31例患者中,nm23暘性錶達22例(71.0%),KAI1暘性錶達19例(61.3%).早期與中晚期患者的nm23和KAI1暘性錶達率差異均有統計學意義(P確切=0.0051,P確切=0.0084),且兩基因暘性率與臨床分期呈負相關(P趨勢=0.0047,P趨勢=0.0058).高、中、低分化膽囊腺癌的nm23和KAI1暘性錶達率差異均有統計學意義(P確切=0.0328,P確切=0.0020),且兩基因暘性率與組織學分級呈正相關(P趨勢=0.0086,P趨勢=0.0006).術後3年,存活(17例)與死亡者(14例)的nm23和KAI1暘性錶達率差異均有統計學意義(P確切=0.0038,P確切=0.0001),存活者中兩基因均錶達暘性15例,而死亡者中兩基因均錶達陰性7例(P確切=0.0005).結論 nm23和KAI1蛋白錶達與膽囊腺癌的臨床分期呈負相關,與組織學分級呈正相關.nm23和KAI1蛋白高錶達預示膽囊腺癌患者預後較好,併且兩基因有協同作用.
목적 탐토종류전이억제기인nm23화KAI1재담낭선암중적표체급기림상의의.방법 수집31례담낭선암환자적병리석사표본화림상병리자료,장표본련속절편진행면역조화염색,검측nm23、KAI1적표체.소유환자수방3년.결과 31례환자중,nm23양성표체22례(71.0%),KAI1양성표체19례(61.3%).조기여중만기환자적nm23화KAI1양성표체솔차이균유통계학의의(P학절=0.0051,P학절=0.0084),차량기인양성솔여림상분기정부상관(P추세=0.0047,P추세=0.0058).고、중、저분화담낭선암적nm23화KAI1양성표체솔차이균유통계학의의(P학절=0.0328,P학절=0.0020),차량기인양성솔여조직학분급정정상관(P추세=0.0086,P추세=0.0006).술후3년,존활(17례)여사망자(14례)적nm23화KAI1양성표체솔차이균유통계학의의(P학절=0.0038,P학절=0.0001),존활자중량기인균표체양성15례,이사망자중량기인균표체음성7례(P학절=0.0005).결론 nm23화KAI1단백표체여담낭선암적림상분기정부상관,여조직학분급정정상관.nm23화KAI1단백고표체예시담낭선암환자예후교호,병차량기인유협동작용.
Objective To investigate the expression of two tumor metastasis suppressor genes nm23and KAI1 in gallbladder adenocarcinoma, and their clinicopathological significance. Methods Specimens and clinical data from 31 gallbladder adenocarcinoma patients were collected. Histopathological grading and the expression of nm23 and KAI1 were detected by HE and immunohistochemical staining, respectively. All cases were followed up for at least three years. Results Immunohistochemical staining showed that the positive rate of nm23 and KAI1 proteins was 71.0% (22/31) and 61.3% (19/31), respectively. The positive expression rates of nm23 and KAI1 proteins in the early stage carcinomas were significantly higher than those in the moderate and advanced stage ones (Pe,x,a,c,t=0.0051 and Pe,x,a,c,t=0.0084), and both had an negative correlation with clinicopathologic stage (Pt,r,e,n,d=0.0047 and Pt,r,e,n,d=0.0058). There was asignificant difference in the expression of nm23 and KAI1 proteins among well, moderately and poorly differentiated carcinomas (Pe,x,a,c,t=0.0328 and Pe,x,a,c,t=0.0020). The expression of nm23 and KAI1 was positively correlated with histopathological grade (Pt,r,e,n,d=0.0086 and Pt,r,e,n,d=0.0006). There was also asignificant difference in the expression of nm23 and KAII proteins between 17 survival and 14 dead patients(Pe,x,a,c,t=0.0038 and Pe,x,a,c,t=0.0001). A synergistic effect of nm23 and KAI1 protein on the survival was observed, and seemed to be more important than any individual gene alone (Pe,x,a,c,t=0.0005). Conclusion The expressions of nm23 and KAI1 proteins are negatively correlated with clinical stage, but positively with histopathological grade in gallbladder adenocarcinoma. These two tumor metastasis suppressor genes may act synergistically to inhibit the tumor metastasis.
