CAJ | 학술논문

微卫星是近年大力开发的一种遗传标记,为推进按蚊遗传学相关研究,对按蚊全基因组中由1～6个碱基重复单元组成的简单序列重复(微卫星)进行了分析.进而对其微卫星的丰度和分布进行了比较分析,也比较了染色体各个区域(外显子、内含子和基因间隔区)之间的分布差异.微卫星在按蚊基因组中的比例约占2.14%,其中X染色体拥有微卫星的密度最大.对按蚊基因组中微卫星丰度而言,A碱基和C碱基重复在基因组中丰度相似,AC单元的丰度是AG单元的两倍多,然而AT和CG单元非常稀少;对于三四碱基而言,AGC,AAAC和AAAT单元最为丰富,ACG,ACT,AGG,CCG,ATGC,CCCG,ACTG,AACT,ACGT,AGAT,CCGG,ACCT和AGCT单元等均很稀少,而一些五碱基重复,在某条甚至某几条染色体中均未分布.除两碱基重复单元在2L的外显子区域丰度较高外,其他重复单元均在内含子和基因间隔区丰富.进一步分析显示,微卫星在每条染色体两臂的丰度和分布存在着很多的相似性.
미위성시근년대력개발적일충유전표기,위추진안문유전학상관연구,대안문전기인조중유1～6개감기중복단원조성적간단서렬중복(미위성)진행료분석.진이대기미위성적봉도화분포진행료비교분석,야비교료염색체각개구역(외현자、내함자화기인간격구)지간적분포차이.미위성재안문기인조중적비례약점2.14%,기중X염색체옹유미위성적밀도최대.대안문기인조중미위성봉도이언,A감기화C감기중복재기인조중봉도상사,AC단원적봉도시AG단원적량배다,연이AT화CG단원비상희소;대우삼사감기이언,AGC,AAAC화AAAT단원최위봉부,ACG,ACT,AGG,CCG,ATGC,CCCG,ACTG,AACT,ACGT,AGAT,CCGG,ACCT화AGCT단원등균흔희소,이일사오감기중복,재모조심지모궤조염색체중균미분포.제량감기중복단원재2L적외현자구역봉도교고외,기타중복단원균재내함자화기인간격구봉부.진일보분석현시,미위성재매조염색체량비적봉도화분포존재착흔다적상사성.
Microsatellite is a genetic marker, explored recently. In order to improve related studies on genetics of Anopheles gambiae, simple sequence repeats of the entire mosquito genome with 1～6 bp nucleotide motifs were analyzed.Abundance and distribution of microsatellites across the A.gambiae genome were analyzed and compared between various (exons, introns and intergenic) regions of all the chromosomes. About 2.14% of the mosquito genome was occupied by SSRs. Chromosome X had the maximum density of SSRs. Abundance orA repeats was similar to C repeats. AC was a little more than two times as abundant much as AG. However, AT and CG repeats were rare. For tri- and tetramer repeats, AGC,AAAC and AAAT predominated while ACG, ACT, AGG, CCG, ATGC, CCCG, ACTG, AACT, ACGT, AGAT, CCGG,ACCT and AGCT were rare. For some pentamer repeats, one was completely absent on a certain chromosome, even on several chromosomes. SSRs in exons of all chromosomes were less abundant than in introns and intergenic regions except for mono- and dimer repeats in exons of chromosome 2L. Abundance and distribution of SSRs on the two arms of each chromosome showed much in common.