CAJ | 학술논문

β-actin是actin家族的一员,在维持细胞结构,细胞内运动,细胞分裂等细胞生理活动方面发挥着重要的作用.克隆的黄鳝β-actin基因的cDNA全长1 860 bp,编码375个氨基酸,在脊椎动物中不同物种的β-actin基因之间的序列同源性超过了98%.RT-PCR表明克隆的黄鳝β-actin基因在睾丸、卵巢、心、肝、脾、脑等组织中广谱表达.基于目前已知的全部鱼类β-actin cds,构建了进化树.星形辐射的树型结构一致支持将鱼类β-actin基因划分为4类.到目前为止,所有的鱼都没有发现拥有全部4个β-actin基因.这暗示伴随着鱼类的辐射式进化历程,可能发生了种系特异性的β-actin的丢失.
β-actin시actin가족적일원,재유지세포결구,세포내운동,세포분렬등세포생리활동방면발휘착중요적작용.극륭적황선β-actin기인적cDNA전장1 860 bp,편마375개안기산,재척추동물중불동물충적β-actin기인지간적서렬동원성초과료98%.RT-PCR표명극륭적황선β-actin기인재고환、란소、심、간、비、뇌등조직중엄보표체.기우목전이지적전부어류β-actin cds,구건료진화수.성형복사적수형결구일치지지장어류β-actin기인화분위4류.도목전위지,소유적어도몰유발현옹유전부4개β-actin기인.저암시반수착어류적복사식진화역정,가능발생료충계특이성적β-actin적주실.
β-actin is a member of the actin family of genes,which play important roles in maintaining cytoskeletal structure, cell motility, cell division, intracellular movements and contractile processes. We report here the identification of a β-actin cDNA of the rice field eel,a teleost fish with a characteristic of natural sex reversal. The cDNA sequence of this gene was 1 860 bp in length,encoding a 375 amino acid protein. Amino acid identities of the β-actin between the rice field eel and other vertebrates including human, chicken, and other fishes, were more than 98%. RT-PCR showed expression of the rice field eel β-actin in testis, ovotestis, ovary, heart, liver,spleen and brain,suggesting a ubiquitous expression pattern. Phylogenetic tree including all β-actin cds of teleost fishes was constructed,which suggested consistently that teleost β-actin can be classified into four types,but no fish has been found contains all the four types of β-actin gene in its genome. The results that suggest lineage-specific β-actin loss might happen in the radical evolution of teleost fishes.