CAJ | 학술논문

已往的研究对于实验室应用的各种啮齿类动物,如大鼠和小鼠骨骼肌蛋白表达的特性已有报道.然而,至今不清楚其它啮齿类动物如野生鼠骨骼肌蛋白的表达或性双态性的特征,而这些野生鼠的行为学、形态学及生理学特点均已有报道.已知骨骼肌的肌球蛋白重链(MHC)成分与其功能特性有关.我们研究了草原田鼠的肱三头肌、胫骨前肌、腓肠肌和比目鱼肌MHC蛋白表达的性别特性.应用SDS聚丙烯酰胺凝胶电泳法测定MHC Ⅰ型、Ⅱa型、Ⅱd/x和Ⅱb型的蛋白表达相对含量.结果表明:与雌鼠相比,雄鼠的比目鱼肌湿重较大,胫骨前肌的MHC Ⅱa蛋白量表达较高.未见骨骼肌重量及MHC蛋白表达含量在雌雄鼠间的性别差异.血中睾酮的浓度差异可能不影响外周骨骼肌蛋白的表达特性.然而,与过去在大鼠、兔和小鼠中的已报道的结果相比,草原田鼠骨骼肌MHC的表达显示了更多异质性.推测这可能与草原田鼠和其它小型哺乳类动物生存的自然环境和功能需要有关.
이왕적연구대우실험실응용적각충교치류동물,여대서화소서골격기단백표체적특성이유보도.연이,지금불청초기타교치류동물여야생서골격기단백적표체혹성쌍태성적특정,이저사야생서적행위학、형태학급생이학특점균이유보도.이지골격기적기구단백중련(MHC)성분여기공능특성유관.아문연구료초원전서적굉삼두기、경골전기、비장기화비목어기MHC단백표체적성별특성.응용SDS취병희선알응효전영법측정MHC Ⅰ형、Ⅱa형、Ⅱd/x화Ⅱb형적단백표체상대함량.결과표명:여자서상비,웅서적비목어기습중교대,경골전기적MHC Ⅱa단백량표체교고.미견골격기중량급MHC단백표체함량재자웅서간적성별차이.혈중고동적농도차이가능불영향외주골격기단백적표체특성.연이,여과거재대서、토화소서중적이보도적결과상비,초원전서골격기MHC적표체현시료경다이질성.추측저가능여초원전서화기타소형포유류동물생존적자연배경화공능수요유관.
Previous research has reported skeletal muscle protein expression characteristics for laboratory strains of various rodents, such as rats and mice. However, we do not know the muscle protein expression or sexual dimorphism characteristics of skeletal muscle for other rodents such as voles, for which the behavior, morphology, and physiology have been documented. Myosin heavy chain (MHC) content in skeletal muscle is related to functional characteristics. This study investigated sex characteristics (male,n=6; female,n=8) for MHC expression for triceps brachii, tibialis anterior, gastrocnemius, and soleus muscles in prairie voles. Relative (%) MHC protein expression was determined via SDS-PAGE for types Ⅰ, Ⅱa, Ⅱd/x, and Ⅱb MHC isoforms. Male voles had greater soleus wet weight and greater Ⅱa MHC expression for tibialis anterior as compared to those of female voles. Skeletal muscle mass and MHC protein expression were not sexually dimorphic. Differences in circulating testosterone titers did not appear to influence these characteristics of mature peripheral skeletal muscle. Prairie voles, however, exhibited much more heterogeneity in MHC expression as compared to previous reports on rats, rabbits, and mice. It is likely that this is due to differences in the natural history and functional requirements of voles and other small mammals.