CAJ | 학술논문

采用聚丙烯酰胺凝胶电泳法(PAGE)对来自6处不同生境的加拿大一枝黄花(Solidago canadensis L.)和栽培品种黄莺(Solidago goldenwings)进行了酯酶同工酶测试、分析;并通过对酶谱特征进行数量化分级,根据欧氏距离及类平均法(UPGMA)对6个供试样品进行聚类分析.结果表明:(1)6个样品都具有2条迁移率分别为0. 62和0. 66的酶带;(2)供试样品间共出现8种不同带型的酶谱表型差异显著,其中不同生境加拿大一枝黄花均聚成一组,显示其酶谱表型与生态型也一致,而栽培品种黄莺则单独成为1组.(3)加拿大一枝黄花与园艺栽培品种黄莺的亲缘关系较远.因此,采用酯酶同工酶测试方法,可以鉴别来自不同生境的加拿大一枝黄花及栽培品种黄莺.
채용취병희선알응효전영법(PAGE)대래자6처불동생경적가나대일지황화(Solidago canadensis L.)화재배품충황앵(Solidago goldenwings)진행료지매동공매측시、분석;병통과대매보특정진행수양화분급,근거구씨거리급류평균법(UPGMA)대6개공시양품진행취류분석.결과표명:(1)6개양품도구유2조천이솔분별위0. 62화0. 66적매대;(2)공시양품간공출현8충불동대형적매보표형차이현저,기중불동생경가나대일지황화균취성일조,현시기매보표형여생태형야일치,이재배품충황앵칙단독성위1조.(3)가나대일지황화여완예재배품충황앵적친연관계교원.인차,채용지매동공매측시방법,가이감별래자불동생경적가나대일지황화급재배품충황앵.
Esterase isozymes in Solidago canadensis L. and S.goldenwings from six habitats were studied using vertical slab polyacrylamide gel electrophoresis. The zymogram variations were significant among those plants. According to the quantitative data of the characteristics of enzyme spectrum, the six plants were systematically clustered using UPGMA method. The results were as follows: (1) all tested plants had two same bands (Rf 0. 62 and Rf 0. 66);(2)eight types of band were presented in the tested plants, all tested plants of S. canadensis were clustered into one group, while the other group only contained one species, S. goldenwings, the plants of S. canadensis collected from the same place were clustered together, showing that their zymogram phenotypes showed almost the same as their ecotype in the region;(3) the phylogenetic relationships of S. canadensis were far away from S. goldenwings. So esterase isozymes could be used as an effective biochemical marker in S. canadensis sampled from various habitats to distinguish S. canadensis from S. goldenwings.