CAJ | 학술논문

在某些蓝藻、绿藻和高等植物中,八氢番茄红素脱氢酶是β-胡萝卜素合成过程中的一个关键酶之一,应用巢式PCR方法从雨生红球藻中克隆了八氢番茄红素脱氢酶基因约1kb的5'上游序列.通过生物信息学方法进行序列分析,发现pds基因上游序列中包含了一些可能的顺式元件,如ABRE 调控元件、C-repeat/DRE调控元件等.同时,构建了由pds-启动子控制报告基因的重组载体,并转化到雨生红球藻细胞中,进行了报告基因瞬间表达的检测.结果表明,克隆的pds启动子区域具有启动子活性,可以驱使报告基因进行瞬间表达.
재모사람조、록조화고등식물중,팔경번가홍소탈경매시β-호라복소합성과정중적일개관건매지일,응용소식PCR방법종우생홍구조중극륭료팔경번가홍소탈경매기인약1kb적5'상유서렬.통과생물신식학방법진행서렬분석,발현pds기인상유서렬중포함료일사가능적순식원건,여ABRE 조공원건、C-repeat/DRE조공원건등.동시,구건료유pds-계동자공제보고기인적중조재체,병전화도우생홍구조세포중,진행료보고기인순간표체적검측.결과표명,극륭적pds계동자구역구유계동자활성,가이구사보고기인진행순간표체.
Phytoene desaturase is one of the most important enzymes necessary for the biosynthesis of carotenoids in some cyanobacteria,green algae and plants.To locate transcriptional regulation elements,we isolated an approximate 1 kb of 5'flanking region by genome-walking method.Results of bioinformatic analysis showed that several putative cis-elements like the ABRE motif(abscisic acid responsive element),the C-repeat/DRE(dehydration responsive element) motif and the GCN4 motif were located in 5'flanking region of pds.Then,we constructed the recombinant plasmid containing the reporter gene lacZ driven by pds promoter and transferred the plasmid into the cells.The transient expression results showed that pds promoter activity can be detected.