CAJ | 학술논문

背景氧化应激能促使糖尿病性白内障的发生,铁螯合剂可以减轻糖尿病患者的氧化应激反应.目的探讨去铁酮对糖尿病性白内障的干预作用.方法收集6周龄雄性Wistar大鼠40只,按随机数字表法分为4个组.正常对照组8只给予普通饲料喂养、腹腔内注射40 mg/kg柠檬酸缓冲液,剩余32只大鼠采用高糖高脂饮食并腹腔内注射40 mg/kg链脲佐菌素(STZ)法建立糖尿病模型,50 mg去铁酮组、100 mg去铁酮组分别给予50 mg/kg和100 mg/kg的去铁酮溶液灌胃,每日1次,共持续8周；糖尿病对照组造模后不做干预.于实验第8周用考马斯亮蓝测定各组大鼠晶状体中水溶性蛋白(WSP)、脲溶性蛋白(USP)和碱溶性蛋白(ASP)的质量浓度,分别用黄嘌呤氧化酶法、硫代巴比妥酸法和二硫代二硝基苯甲酸法测定晶状体匀浆中超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量和谷胱甘肽(GSH)的质量分数.结果各组大鼠晶状体中的WSP、USP和ASP质量浓度的差异均无统计学意义(F=1.73、0.18、0.09,P＞0.05).50 mg去铁酮组和100 mg去铁酮组大鼠晶状体匀浆中MDA含量分别为(1.05±0.10) mmol/g、( 1.05±0.22) mmol/g,均低于糖尿病对照组的( 1.49±0.38) mmol/g,差异均有统计学意义(P＜0.05).50 mg去铁酮组和100 mg去铁酮组大鼠晶状体匀浆中SOD活性和GSH质量分数分别为(321.29±16.57) U/mg、(322.07± 22.16)U/mg和(7.83±0.65) mg/g、(7.70±0.77) mg/g,明显高于糖尿病组的(298.70±14.69) U/mg及(5.47±1.01) mg/g,差异均有统计学意义(P＜0.05).50 mg去铁酮组和100 mg去铁酮组间上述各项指标间的差异均无统计学意义(P＞0.05).结论去铁酮可以减轻晶状体的氧化应激反应,改善晶状体的能量代谢.
배경 양화응격능촉사당뇨병성백내장적발생,철오합제가이감경당뇨병환자적양화응격반응.목적 탐토거철동대당뇨병성백내장적간예작용.방법 수집6주령웅성Wistar대서40지,안수궤수자표법분위4개조.정상대조조8지급여보통사료위양、복강내주사40 mg/kg저몽산완충액,잉여32지대서채용고당고지음식병복강내주사40 mg/kg련뇨좌균소(STZ)법건립당뇨병모형,50 mg거철동조、100 mg거철동조분별급여50 mg/kg화100 mg/kg적거철동용액관위,매일1차,공지속8주；당뇨병대조조조모후불주간예.우실험제8주용고마사량람측정각조대서정상체중수용성단백(WSP)、뇨용성단백(USP)화감용성단백(ASP)적질량농도,분별용황표령양화매법、류대파비타산법화이류대이초기분갑산법측정정상체균장중초양화물기화매(SOD)활성이급병이철(MDA)함량화곡광감태(GSH)적질량분수.결과 각조대서정상체중적WSP、USP화ASP질량농도적차이균무통계학의의(F=1.73、0.18、0.09,P＞0.05).50 mg거철동조화100 mg거철동조대서정상체균장중MDA함량분별위(1.05±0.10) mmol/g、( 1.05±0.22) mmol/g,균저우당뇨병대조조적( 1.49±0.38) mmol/g,차이균유통계학의의(P＜0.05).50 mg거철동조화100 mg거철동조대서정상체균장중SOD활성화GSH질량분수분별위(321.29±16.57) U/mg、(322.07± 22.16)U/mg화(7.83±0.65) mg/g、(7.70±0.77) mg/g,명현고우당뇨병조적(298.70±14.69) U/mg급(5.47±1.01) mg/g,차이균유통계학의의(P＜0.05).50 mg거철동조화100 mg거철동조간상술각항지표간적차이균무통계학의의(P＞0.05).결론 거철동가이감경정상체적양화응격반응,개선정상체적능량대사.
Background Oxidative stress is thought to be responsible to diabetes-complicated cataract.Our previous study demonstrated that as an iron chelator,deferiprone can protect lens from oxidative damage.Objective This further study aimed to investigate the role of deferiprone on the formation of diabetic-complicated cataract.Methods Forty 6-week-old Wistar rats were included in the study and randomized into 4 groups.Eight of them were used as the normal control group.Diabetes mellitus animal models were established in 22 rats by the carbonhydratediet and fat diet and the intraperitoneal injection of 40 mg/kg streptozocin (STZ).The deferiprone of 50 mg and 100 mg were intragastrically given in 8 model rats respectively after 3 days once a day for 8 weeks.The opacification of lenses was examined under the slit lamp weekly after treatment.The animals were sacrificed and the lenses were obtained at the eighth week of deferiprone injection.The concentrations of water-soluble protein ( WSP),urine-soluble protein (USP) and alkali-soluble protein (ASP) in rat lens suspension were detected by Bradford method.The super oxide dimutese (SOD),malondialdehyde (MDA) and glutathione (GSH) were determined spectrometically using xanthine oxidase,thiobarbituric acid,dithio bis-nitrobenzoic acid.Results No evidently differences were found in the content of the WSP,USP and ASP among the these groups( F=1.73,0.18,0.09,P＞0.05).The contents of MDA in 50 mg deferiprone group and 100 mg deferiprone group were ( 1.05 ± 0.10 ) mmol/g and ( 1.05 ± 0.22 ) mmol/g respectively,showing a significant decline in comparison with diabetic model group (P＜0.05).The SOD and GSH contents in lens were (321.29±16.57) U/mg,(322.07±22.16) U/mg and (7.83±0.65 ) mg/g,(7.70±0.77 ) mg/g respectively in 50 mg deferiprone group and 100 mg deferiprone group and were considerably elevated in comparison with ( 298.70± 14.69 ) U/mg and ( 5.47 ± 1.01 ) mg/g of diabetic model groups ( P＜0.05 ).No significant differences were found in the indexes mentioned above between 50 mg and 100 mg deferiprone groups(P＞0.05).Conclusions Deferiprone can reduce oxidative stress and improve the energy metabolism of the lens in diabetic rats.