CAJ | 학술논문

目的探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中增殖诱导配体(APRIL)及其受体B细胞成熟抗原(BCMA)、跨膜激活剂及钙调亲环素配体相互作用分子(TACI)mRNA的表达和意义.方法应用实时定量聚合酶链反应(real-time PCR)技术,检测66例SLE患者及25名正常人PBMC中APRIL及其受体mRNA的表达,采用2-AACT对目标基因的表达量进行评估.结果 SLE活动组和缓解组的APRIL mRNA、BCMA mRNA和TACI mRNA的表达水平均明显高于正常对照组(均P<0.01);且SLE活动组的APRIL mRNA和TACI mRNA的表达水平显著高于SLE缓解组(分别P<0.01,P<0.05),BCMA mRNA的表达在SLE活动组与缓解组之间比较差异无统计学意义;此外,APRILmRNA和TACI mRNA在SLE肾损组的表达显著高于非肾损组(均P<0.01).结论 SLE患者PBMC中APRIL及其受体的表达水平增高,可能在SLE发病机制中发挥重要作用.
목적 탐토계통성홍반랑창(SLE)환자외주혈단개핵세포(PBMC)중증식유도배체(APRIL)급기수체B세포성숙항원(BCMA)、과막격활제급개조친배소배체상호작용분자(TACI)mRNA적표체화의의.방법 응용실시정량취합매련반응(real-time PCR)기술,검측66례SLE환자급25명정상인PBMC중APRIL급기수체mRNA적표체,채용2-AACT대목표기인적표체량진행평고.결과 SLE활동조화완해조적APRIL mRNA、BCMA mRNA화TACI mRNA적표체수평균명현고우정상대조조(균P<0.01);차SLE활동조적APRIL mRNA화TACI mRNA적표체수평현저고우SLE완해조(분별P<0.01,P<0.05),BCMA mRNA적표체재SLE활동조여완해조지간비교차이무통계학의의;차외,APRILmRNA화TACI mRNA재SLE신손조적표체현저고우비신손조(균P<0.01).결론 SLE환자PBMC중APRIL급기수체적표체수평증고,가능재SLE발병궤제중발휘중요작용.
Objective To investigate the mRNA expression of a proliferation inducing ligand (APRIL) and its receptors including B cell maturation antigen (BCMA),transmembrane activator.calcium modulator and cyclophilin ligand interactor (TACI) in the peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SEE).Methods APRIL mRNA、BCMA mRNA and TACI mRNA in PBMCs were detected by real-time quantitative PCR in 66 SLE patients and 25 normal controls.Gene expression level was measured as 2-AACT.Results The expression levels of APRIL mRNA、BCMA mRNA and TACI-mRNA were significantly increased in both active SLE group and stable SLE group compared with those in the normal controls(P<0.01 for all).The expression levels of APRIL mRNA and TACI mRNA in active SLE group were significantly higher than those in stable SLE group(P<0.01,P<0.05,respectively).But there was no significant difierence in the expression levels of BCMA mRNA between the SLE stable and active groups-Beside,the expression levels of APRIL mRNA and TACI mRNA were significantly increased in patients with lupus nephritis (LN) compared to patients with non-LN (P<0.01 for all).Conclusion The expression levels of APRIL and its receptors are significantly elevated in SLE patients.It may suggest that APRIL and its receptors play an important role in the pathogenesis of SLE.