中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2009年
7期
577-579
,共3页
范郑丽%隋建峰%李蕊%万子兵%赵红梅
範鄭麗%隋建峰%李蕊%萬子兵%趙紅梅
범정려%수건봉%리예%만자병%조홍매
眨眼条件反射%豚鼠%声音%光%习得率
眨眼條件反射%豚鼠%聲音%光%習得率
잡안조건반사%돈서%성음%광%습득솔
Eyeblink conditioned reflex%Guinea pig%Tone%Light%Acquisition rate
目的 以眼肌电记录法分别记录豚鼠以声音和光信号作为条件刺激(CS)的眨眼条件反射(CR),分析并比较其习得规律.方法 采用金属电极埋植技术及简易"随动"气流给气刺激装置,分别以声音和光信号作为条件刺激(CS),以气流作为非条件刺激(US),以眼肌电信号判断其眨眼行为,在清醒无制动的豚鼠上进行经典眨眼条件反射的训练.结果 在豚鼠内眦采集并记录到眨眼肌电信号显示,声音条件刺激组在第1天、第4天、第7天、第10天的CR习得率分别为(5.0±1.09)%、(31.0±2.09)%、(61.33±1.63)%、(82.33±1.63)%,而光条件刺激组在第1天、第4天、第7天、第10天的CR习得率分别为(5.5±4.59)%、(4.33±3.14)%、(10.83±13.42)%、(3.83±1.33)%,2组CR习得率差异有显著性(P<0.05).结论 以声音作为条件刺激时豚鼠能够成功建立的眨眼条件反射,而以光作为条件刺激时眨眼条件反射的建立却非常困难.
目的 以眼肌電記錄法分彆記錄豚鼠以聲音和光信號作為條件刺激(CS)的眨眼條件反射(CR),分析併比較其習得規律.方法 採用金屬電極埋植技術及簡易"隨動"氣流給氣刺激裝置,分彆以聲音和光信號作為條件刺激(CS),以氣流作為非條件刺激(US),以眼肌電信號判斷其眨眼行為,在清醒無製動的豚鼠上進行經典眨眼條件反射的訓練.結果 在豚鼠內眥採集併記錄到眨眼肌電信號顯示,聲音條件刺激組在第1天、第4天、第7天、第10天的CR習得率分彆為(5.0±1.09)%、(31.0±2.09)%、(61.33±1.63)%、(82.33±1.63)%,而光條件刺激組在第1天、第4天、第7天、第10天的CR習得率分彆為(5.5±4.59)%、(4.33±3.14)%、(10.83±13.42)%、(3.83±1.33)%,2組CR習得率差異有顯著性(P<0.05).結論 以聲音作為條件刺激時豚鼠能夠成功建立的眨眼條件反射,而以光作為條件刺激時眨眼條件反射的建立卻非常睏難.
목적 이안기전기록법분별기록돈서이성음화광신호작위조건자격(CS)적잡안조건반사(CR),분석병비교기습득규률.방법 채용금속전겁매식기술급간역"수동"기류급기자격장치,분별이성음화광신호작위조건자격(CS),이기류작위비조건자격(US),이안기전신호판단기잡안행위,재청성무제동적돈서상진행경전잡안조건반사적훈련.결과 재돈서내자채집병기록도잡안기전신호현시,성음조건자격조재제1천、제4천、제7천、제10천적CR습득솔분별위(5.0±1.09)%、(31.0±2.09)%、(61.33±1.63)%、(82.33±1.63)%,이광조건자격조재제1천、제4천、제7천、제10천적CR습득솔분별위(5.5±4.59)%、(4.33±3.14)%、(10.83±13.42)%、(3.83±1.33)%,2조CR습득솔차이유현저성(P<0.05).결론 이성음작위조건자격시돈서능구성공건립적잡안조건반사,이이광작위조건자격시잡안조건반사적건립각비상곤난.
Objective To record the classical eyeblink conditioning single,which the conditioned stimu-lus(CS) is tone or light, by the electromyographic (EMG) of musculi oculi recording. Methods Adopting metal electrode embedding technic and simple air puff stimulus facility ,tone or light as the conditioned stimulus(CS) was paired with air puff being unconditioned stimulus(US) to establish classical eyeblink conditioning of conscious un-fixed guinea pigs. The eyeblink behavior were evaluated through EMG of musculi oculi. Results The eyeblink EMG signal of musculi oculi showed: when the tone as the CS , the results indicated that the acquisition rate of the 1st,the 4th,the 7th,the 10th day were respectively (5.0±1.09)%,(31.0±2.09)%,(61.33±1.63)%,(82.33±1.63) % ; when the light as the CS , the results indicate that the acquisition rate of the 1 st, the 4th, the 7th, the 10th day were respectively (5.5±4.59) %, (4.33±3.14) %, (10.83±13.42)%, (3.83±1.33 ) %. Conclu-sion While CS is tone,classical eyeblink conditioning can be successfully established for the guinea pigs;on an-other hand, while CS is light,it is very hard for the guinea pigs to establish the classical eyeblink conditioning.
