中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2009年
5期
460-462
,共3页
麻醉药%吸入%缺血预处理%内毒素血症%呼吸窘迫综合征%成人
痳醉藥%吸入%缺血預處理%內毒素血癥%呼吸窘迫綜閤徵%成人
마취약%흡입%결혈예처리%내독소혈증%호흡군박종합정%성인
Anesthetics,inhalation%lschemic preconditioning%Eudotoxemia%Respiratory distress syndrome,adult
目的 探讨七氟醚预处理对大鼠内毒素性急性肺损伤的影响.方法 健康雄性SD大鼠24只,体重220～250 g,随机分为4组(n=6):对照组(c组)、内毒素组(LPS组)、七氟醚组(Sev组)和七氟醚预处理组(SP组).C组和LPS组机械通气30 min后分别静脉注射生理盐水和脂多糖(LPS)5 mg/kg;Sev组和SP组吸入七氟醚(呼气末浓度2.4%)30 min,洗脱5 min,然后分别静脉注射生理盐水和LPS 5 mg/kg.于给予LPS或生理盐水后6 h时,心脏放血处死大鼠,取肺组织,计算湿重/干重(W/D)比;采用弥漫性肺泡损伤(DAD)评分评价肺组织损伤程度;测定肺组织髓过氧化物酶(MPO)活性、细胞因子诱导中性粒细胞化学趋化因子-1(CINC-1)含量、CINC-1和CINC-1 mRNA的表达水平.结果 与C组比较,LPS组和SP组肺组织W/D比、DAD评分、MPO活性和CINC-1含量升高,CINC-1和CINC-1 mRNA的表达上调(P<0.01),Sev组上述指标差异无统计学意义(P>0.05);与LPS组比较,SP组肺组织W/D比、DAD评分、MPO活性和CINC-1含量降低,CINC-1和CINC-1 mRNA的表达下调(P<0.01).结论 七氟醚预处理可减轻大鼠内毒素性急性肺损伤,可能与其抑制肺组织炎性反应有关.
目的 探討七氟醚預處理對大鼠內毒素性急性肺損傷的影響.方法 健康雄性SD大鼠24隻,體重220～250 g,隨機分為4組(n=6):對照組(c組)、內毒素組(LPS組)、七氟醚組(Sev組)和七氟醚預處理組(SP組).C組和LPS組機械通氣30 min後分彆靜脈註射生理鹽水和脂多糖(LPS)5 mg/kg;Sev組和SP組吸入七氟醚(呼氣末濃度2.4%)30 min,洗脫5 min,然後分彆靜脈註射生理鹽水和LPS 5 mg/kg.于給予LPS或生理鹽水後6 h時,心髒放血處死大鼠,取肺組織,計算濕重/榦重(W/D)比;採用瀰漫性肺泡損傷(DAD)評分評價肺組織損傷程度;測定肺組織髓過氧化物酶(MPO)活性、細胞因子誘導中性粒細胞化學趨化因子-1(CINC-1)含量、CINC-1和CINC-1 mRNA的錶達水平.結果 與C組比較,LPS組和SP組肺組織W/D比、DAD評分、MPO活性和CINC-1含量升高,CINC-1和CINC-1 mRNA的錶達上調(P<0.01),Sev組上述指標差異無統計學意義(P>0.05);與LPS組比較,SP組肺組織W/D比、DAD評分、MPO活性和CINC-1含量降低,CINC-1和CINC-1 mRNA的錶達下調(P<0.01).結論 七氟醚預處理可減輕大鼠內毒素性急性肺損傷,可能與其抑製肺組織炎性反應有關.
목적 탐토칠불미예처리대대서내독소성급성폐손상적영향.방법 건강웅성SD대서24지,체중220～250 g,수궤분위4조(n=6):대조조(c조)、내독소조(LPS조)、칠불미조(Sev조)화칠불미예처리조(SP조).C조화LPS조궤계통기30 min후분별정맥주사생리염수화지다당(LPS)5 mg/kg;Sev조화SP조흡입칠불미(호기말농도2.4%)30 min,세탈5 min,연후분별정맥주사생리염수화LPS 5 mg/kg.우급여LPS혹생리염수후6 h시,심장방혈처사대서,취폐조직,계산습중/간중(W/D)비;채용미만성폐포손상(DAD)평분평개폐조직손상정도;측정폐조직수과양화물매(MPO)활성、세포인자유도중성립세포화학추화인자-1(CINC-1)함량、CINC-1화CINC-1 mRNA적표체수평.결과 여C조비교,LPS조화SP조폐조직W/D비、DAD평분、MPO활성화CINC-1함량승고,CINC-1화CINC-1 mRNA적표체상조(P<0.01),Sev조상술지표차이무통계학의의(P>0.05);여LPS조비교,SP조폐조직W/D비、DAD평분、MPO활성화CINC-1함량강저,CINC-1화CINC-1 mRNA적표체하조(P<0.01).결론 칠불미예처리가감경대서내독소성급성폐손상,가능여기억제폐조직염성반응유관.
Objective To investigate the effects of sevoflurane preconditioning (SP) on acute lung injury induced by lipopolysaccharide (LPS) in rats.Methods Twenty-four male SD rats weighing 220-250 g were randomly divided into 4 groups (n = 6 each): group Ⅰ control (group C),group Ⅱ LPS,group Ⅲ sevoflurane (group Sev) and group Ⅳ SP + LPS.In group Ⅰ and Ⅱ ,normal saline and LPS 5 mg/kg were given Ⅳ 30 min after ventilation respectively.In group Ⅲ and Ⅳ,the animals inhaled sevoflurane (end-tidal concentration 2.4% ) for 30 min followed by 5 min wash-out,and then received iv injection of normal saline and LPS 5 mg/kg respectively.The animals were killed at 6 h after LPS or normal saline administration.Lungs were removed for determination of W/D lung weight ratio,myeloperoxidase (MPO) activity,cytokine-induced neutrophil chemoattractant-1 (CINC-1) content and expression of CINC-1 and CINC-1 mRNA.The severity of lung injury was evaluated using diffuse alveolar damage (DAD) score.Results Compared with group Ⅰ ,W/D lung weight ratio,DAD score,MPO activity and CINC-1 content were significantly increased,and expression of CINC-1 and CINC-1 mRNA up-regulated in group Ⅱ and Ⅲ,while there was no significant difference in the above indices between group Ⅲ and group Ⅰ .Compared with group Ⅱ ,W/D lung weight ratio,DAD score,MPO activity and CINC-1 content were significantly decreased,and expression of CINC-1 and CINC-1 mRNA down-regulated in group Ⅲ.Conclusion Sevoflurane preconditioning can protect the lungs against LPS-induced acute lung injury by inhibiting inflaunnatory response.
