CAJ | 학술논문

目的了解兰州地区腹泻患儿中杯状病毒和腺病毒感染的分子流行病学及临床特点.方法收集兰州大学第一医院2010年7月至2011年6月腹泻患儿粪便标本295份,采用RT-PCR或PCR的方法检测杯状病毒及腺病毒,腺病毒阳性标本利用多重PCR及巢式PCR的方法分型,并对序列进行分析.结果 295份粪便标本中杯状病毒的检出率为13.2％( 39/295),腺病毒的检出率是5.1％ (15/295).分型结果显示:杯状病毒中69.2％为诺如病毒,其余是札如病毒,诺如病毒中以GⅡ-3(13例)为主,其次为GⅡ-4(12例),GⅡ-6(2例);腺病毒主要以F组的41型(10/15)为主,同时还检测到1例A组的31型,2例B组的3型及C组的1例5型和1例6型,两种病毒均主要感染2岁以下儿童,无明显的季节高峰.结论杯状病毒和腺病毒是2010 -2011年兰州地区病毒性腹泻患儿的重要病原,长期监测具有重要意义.
목적 료해란주지구복사환인중배상병독화선병독감염적분자류행병학급림상특점.방법 수집란주대학제일의원2010년7월지2011년6월복사환인분편표본295빈,채용RT-PCR혹PCR적방법검측배상병독급선병독,선병독양성표본이용다중PCR급소식PCR적방법분형,병대서렬진행분석.결과 295빈분편표본중배상병독적검출솔위13.2％( 39/295),선병독적검출솔시5.1％ (15/295).분형결과현시:배상병독중69.2％위낙여병독,기여시찰여병독,낙여병독중이GⅡ-3(13례)위주,기차위GⅡ-4(12례),GⅡ-6(2례);선병독주요이F조적41형(10/15)위주,동시환검측도1례A조적31형,2례B조적3형급C조적1례5형화1례6형,량충병독균주요감염2세이하인동,무명현적계절고봉.결론 배상병독화선병독시2010 -2011년란주지구병독성복사환인적중요병원,장기감측구유중요의의.
Objective To investigate the clinical and molecular epidemiology characteristics of calicivirus and adenovirus in children for viral diarrhea in Lanzhou.Methods Stool samples were collected from 295 children with diarrhea at the First Hospital of Lanzhou University,Gansu Province,China,between July 2010 and June 2011.Reverse transcription-polymerase chain reaction (RT-PCR) or PCR were used to detected calicivirus and adenovirus.The adenovirus positive samples were typed by nested PCR and multiple PCR.Results Of the 295 specimens,13.2％ ( 39/295 ) were positive for calicivirus,and 5.1％ ( 15/295 ) were adenovirus.Typing and Phylogenetic analysis revealed that novirus GⅡ-3 and adenovirus 41 were the dominant strains.Both calicivirus and adenovirus predominately affect children under the age of 2.In seasonal distribution,there was no obvious peak.Conclusion Human calicivirus and adenovirus are important pathogens of viral diarrhea,it is important to develop long-term systematic surveillance.