CAJ | 학술논문

目的检测黑色素瘤抗原(MAGE) -D4基因在非小细胞肺癌(NSCLC)组织及正常组织中mRNA的表达水平差异.方法对54例NSCLC患者按年龄、性别、吸烟史、肿瘤大小、病理类型、细胞分化程度、淋巴结有否转移等进行分组,并检测其MAGE-D4的表达.结果 MAGE-D4在NSCLC组织的表达(1.74)高于其在正常组织中的表达(0.55,P＜0.05).MAGE-D4的表达与患者年龄、性别、烟龄、肿瘤大小无关；其在鳞癌中的表达(1.99)高于腺癌(1.27,P＜0.05)；在细胞分化差的组织中表达(2.96)高于细胞分化较好的组织(1.01,P＜0.05)；在淋巴结转移者中的表达(2.07)高于无淋巴结转移者(1.42,P＜0.05).结论 MAGE-D4基因在NSCLC组织中有较高的表达水平,并存在一定的表达规律,可作为NSCLC的特异性抗原.
목적 검측흑색소류항원(MAGE) -D4기인재비소세포폐암(NSCLC)조직급정상조직중mRNA적표체수평차이.방법 대54례NSCLC환자안년령、성별、흡연사、종류대소、병리류형、세포분화정도、림파결유부전이등진행분조,병검측기MAGE-D4적표체.결과 MAGE-D4재NSCLC조직적표체(1.74)고우기재정상조직중적표체(0.55,P＜0.05).MAGE-D4적표체여환자년령、성별、연령、종류대소무관；기재린암중적표체(1.99)고우선암(1.27,P＜0.05)；재세포분화차적조직중표체(2.96)고우세포분화교호적조직(1.01,P＜0.05)；재림파결전이자중적표체(2.07)고우무림파결전이자(1.42,P＜0.05).결론 MAGE-D4기인재NSCLC조직중유교고적표체수평,병존재일정적표체규률,가작위NSCLC적특이성항원.
Objective To study the differential expression of melanoma antigen (MAGE)-D4 gene in non-small cell lung caner (NSCLC) and normal lung tissue.Methods Fifty-four NSCLC patients were classified by age,sex,smoke history,tumor size,pathologic type,cytodifferentiation,lymphatic metastssis.MAGE-D4 gene expression patterns were detected in NSCLC.Results The expression of MAGE-D4 gene in NSCLC was higher than that in normal lung tissue ( 1.74 vs 0.55,P ＜ 0.05 ).The expression level has no relationship with age,sex,smoke history and tumor size.The squamous cell carcinoma had a higer expression than adenocacinoma ( 1.99 vs 1.27,P ＜ 0.05 ).The expression in poorly differentiated tumor was higher than that in moderately differentiated tumor ( 2.96 vs 1.01 P ＜ 0.05 ).The expression in NSCLC with lymphatic metastasis was higher than in that without lymphatic metastasis (2.07 vs 1.42,P ＜0.05 ).Conclusion MAGE-D4 gene has a higher expression level in NSCLC and its expression may follow a certain rule.The MAGE-D4 gene could be potentially a specific NSCLC antigen.