CAJ | 학술논문

目的观察GABA 对谷氨酸和低糖诱导的人少突胶质细胞瘤细胞凋亡增多的影响,并探讨其可能的机制.方法采用免疫组织化学方法和相关的试剂盒测定凋亡百分率的变化和bcl-2、bax、c-fos蛋白的表达.结果①GABA 可以显著抑制谷氨酸和低糖诱导的细胞凋亡百分率的增加(P<0.05);②GABA对谷氨酸和低糖诱导的bcl-2、bax蛋白的表达增加无明显影响;③谷氨酸,低糖和GABA对c-fos蛋白的表达没有明显影响.结论①GABA可能通过降低细胞凋亡的百分率而具有神经保护作用;②GABA的神经保护作用可能是通过其它途径而与bcl-2、bax蛋白的表达无关.
목적관찰GABA 대곡안산화저당유도적인소돌효질세포류세포조망증다적영향,병탐토기가능적궤제.방법채용면역조직화학방법화상관적시제합측정조망백분솔적변화화bcl-2、bax、c-fos단백적표체.결과①GABA 가이현저억제곡안산화저당유도적세포조망백분솔적증가(P<0.05);②GABA대곡안산화저당유도적bcl-2、bax단백적표체증가무명현영향;③곡안산,저당화GABA대c-fos단백적표체몰유명현영향.결론①GABA가능통과강저세포조망적백분솔이구유신경보호작용;②GABA적신경보호작용가능시통과기타도경이여bcl-2、bax단백적표체무관.
Objective To observe the effect of GABA on increased apoptosis of human oligodendroglioma cell (HOC) induced by glutamate (Glu) and low glucose (LG) and find out the potential mechanism.Methods Immunohistochemical staining methods and the kits concerned were employed to examine changes of apoptotic percentage and expression of bcl-2, bax and c-fos proteins.Results ①GABA inhibited the increase of percentage of HOC apoptotic death mediated by Glu and LG (P<0.05);②GABA had no marked effect on the overexpression of bcl-2 and bax proteins induced by Glu and LG;③After 24h treatment, Glu and LG didn't regulate the expression of c-fos protein and GABA also didn't have influence on it.Conclusion ①GABA may have neuroprotection through inhibiting the increased amount of apoptotic cell death mediated by Glu and LG;②The potential mechanism of neuroprotective effect of GABA isn't related to bcl-2 and bax proteins.