中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2008年
14期
2773-2776
,共4页
马艳%岳秀丽%刘萌%刘绍琴%戴志飞
馬豔%嶽秀麗%劉萌%劉紹琴%戴誌飛
마염%악수려%류맹%류소금%대지비
膨体聚四氟乙烯人工血管%共价交联%抗凝血表面修饰%血液相容性
膨體聚四氟乙烯人工血管%共價交聯%抗凝血錶麵脩飾%血液相容性
팽체취사불을희인공혈관%공개교련%항응혈표면수식%혈액상용성
背景:小口径(直径小于6 mm)膨体聚四氟乙烯人工血管由于血栓形成和内膜增生等原因,在冠状和外周血液循环中的应用受到了限制,而生物活性表面肝素涂层是解决上述问题的一条有效途径.目的:为了提高膨体聚四氟乙烯人工血管的抗凝血性能,采用新型肝素涂层--共价交联的聚乙烯醇/重氮树脂/肝素凝胶修饰小口径膨体聚四氟乙烯人工血管以提高其通畅性,并观察其血液相容性.设计、时间及地点:观察性实验,于2006-05/2007-06在哈尔滨工业大学生物医学工程中心的纳米医药与生物传感器实验室完成.材料:膨体聚四氟乙烯人工血管(直径4 mm),全氟磺酸和聚乙烯醇购于美国Aldrich公司,肝素购于Calbiochem公司(Mw12 000～14 000),重氮树脂由实验室自己合成.方法:①用全氟磺酸修饰膨体聚四氟乙烯表面.②用聚乙烯醇/重氦树脂/肝素凝胶进行灌注修饰,在紫外光照射的条件下,重氮树脂作为交联剂,将肝素和聚乙烯醇分子进行共价交联.主要观察指标:①接触角.②衰减全反射-傅里叶变换红外光谱表征材料表面的化学结构.⑨活化部分凝血激酶时间、凝血酶原时间.④溶血试验.⑤血小板黏附试验.⑥凝血酶失活试验.结果:①修饰后的人工血管的接触角降低,衰减全反射-傅立叶变换红外光谱显示在2 172 cm-1和2 224 cm-1处出现了重氮基团的特征峰位.②与未修饰的人工血管相比,修饰后的人工血管具有较长的活化部分凝血激酶时间和凝血酶原时间、较低的溶血度、很少数量的血小板黏附.③与未修饰的人工血管相比,修饰后的人工血管对凝血酶的活性有较强的抑制作用,且涂层稳定.结论:聚乙烯醇/重氮树脂/肝素凝胶形成的肝素涂层不仅具有很好的抗血栓形成的性能,而且具有较低的溶血度,因此显著提高了人工血管的血液相容性.
揹景:小口徑(直徑小于6 mm)膨體聚四氟乙烯人工血管由于血栓形成和內膜增生等原因,在冠狀和外週血液循環中的應用受到瞭限製,而生物活性錶麵肝素塗層是解決上述問題的一條有效途徑.目的:為瞭提高膨體聚四氟乙烯人工血管的抗凝血性能,採用新型肝素塗層--共價交聯的聚乙烯醇/重氮樹脂/肝素凝膠脩飾小口徑膨體聚四氟乙烯人工血管以提高其通暢性,併觀察其血液相容性.設計、時間及地點:觀察性實驗,于2006-05/2007-06在哈爾濱工業大學生物醫學工程中心的納米醫藥與生物傳感器實驗室完成.材料:膨體聚四氟乙烯人工血管(直徑4 mm),全氟磺痠和聚乙烯醇購于美國Aldrich公司,肝素購于Calbiochem公司(Mw12 000～14 000),重氮樹脂由實驗室自己閤成.方法:①用全氟磺痠脩飾膨體聚四氟乙烯錶麵.②用聚乙烯醇/重氦樹脂/肝素凝膠進行灌註脩飾,在紫外光照射的條件下,重氮樹脂作為交聯劑,將肝素和聚乙烯醇分子進行共價交聯.主要觀察指標:①接觸角.②衰減全反射-傅裏葉變換紅外光譜錶徵材料錶麵的化學結構.⑨活化部分凝血激酶時間、凝血酶原時間.④溶血試驗.⑤血小闆黏附試驗.⑥凝血酶失活試驗.結果:①脩飾後的人工血管的接觸角降低,衰減全反射-傅立葉變換紅外光譜顯示在2 172 cm-1和2 224 cm-1處齣現瞭重氮基糰的特徵峰位.②與未脩飾的人工血管相比,脩飾後的人工血管具有較長的活化部分凝血激酶時間和凝血酶原時間、較低的溶血度、很少數量的血小闆黏附.③與未脩飾的人工血管相比,脩飾後的人工血管對凝血酶的活性有較彊的抑製作用,且塗層穩定.結論:聚乙烯醇/重氮樹脂/肝素凝膠形成的肝素塗層不僅具有很好的抗血栓形成的性能,而且具有較低的溶血度,因此顯著提高瞭人工血管的血液相容性.
배경:소구경(직경소우6 mm)팽체취사불을희인공혈관유우혈전형성화내막증생등원인,재관상화외주혈액순배중적응용수도료한제,이생물활성표면간소도층시해결상술문제적일조유효도경.목적:위료제고팽체취사불을희인공혈관적항응혈성능,채용신형간소도층--공개교련적취을희순/중담수지/간소응효수식소구경팽체취사불을희인공혈관이제고기통창성,병관찰기혈액상용성.설계、시간급지점:관찰성실험,우2006-05/2007-06재합이빈공업대학생물의학공정중심적납미의약여생물전감기실험실완성.재료:팽체취사불을희인공혈관(직경4 mm),전불광산화취을희순구우미국Aldrich공사,간소구우Calbiochem공사(Mw12 000～14 000),중담수지유실험실자기합성.방법:①용전불광산수식팽체취사불을희표면.②용취을희순/중양수지/간소응효진행관주수식,재자외광조사적조건하,중담수지작위교련제,장간소화취을희순분자진행공개교련.주요관찰지표:①접촉각.②쇠감전반사-부리협변환홍외광보표정재료표면적화학결구.⑨활화부분응혈격매시간、응혈매원시간.④용혈시험.⑤혈소판점부시험.⑥응혈매실활시험.결과:①수식후적인공혈관적접촉각강저,쇠감전반사-부립협변환홍외광보현시재2 172 cm-1화2 224 cm-1처출현료중담기단적특정봉위.②여미수식적인공혈관상비,수식후적인공혈관구유교장적활화부분응혈격매시간화응혈매원시간、교저적용혈도、흔소수량적혈소판점부.③여미수식적인공혈관상비,수식후적인공혈관대응혈매적활성유교강적억제작용,차도층은정.결론:취을희순/중담수지/간소응효형성적간소도층불부구유흔호적항혈전형성적성능,이차구유교저적용혈도,인차현저제고료인공혈관적혈액상용성.
BACKGROUND:Thrombus formation and neointimal hyperplasia still limit the use of small-caliber expanded poly(tetrafluoroethylene) (ePTFE) vascular prosthesis with a diameter less than 6 mm for revascularization in the coronary or peripheral circulation. Bioactive surface heparin coating is one conceivable path for above-mentioned problems.OBJECTIVE: To elevate the anticoagulant property of ePTFE, this study promoted the patency of a novel small-caliber ePTFE vascular graft by modifying its luminal surface with covalently crosslinked poly(vinyl alcohol)/ p-diazonium diphenyl amine polymer/heparin gel (PVA/PA/Hep gel) and examined the hemocompatibility of the graft.DESIGN, TIME AND SETTING: Observational experiments were performed at the Nanomedicine and Biosensor Lab,Biomedical Engineering Center, Harbin Institute of Technology from May 2006 to June 2007.MATERIALS: The ePTFE vascular grafts (diameter of 4 mm), Nafion (Naf) and Poly(vinyl alcohol) (Aldrich, USA), heparin (Mw 12 000- 14 000) (Calbiochem, USA), p-diazonium diphenyl amine polymer (PA) (this lab, China) were used in this study.METHODS: ①The vascular graft surface was firstly modified with Nafion. ②Following the impregnation of the mixture of PVA/PA/Hep, covalent crosslinking between polyvinyl alcohol and heparin was performed using crosslinker PA under ultraviolet radiation.MAIN OUTCOME MEASURES: ①Contact angles, ②Attenuated total reflection-fourier transform infrared spectroscopy (ATR-FTIR), ③Activated partial thromboplastin time (APTT) and prothromhin time (PT), ④hemolysis test, ⑤platelet adhesion test and ⑥thrombosin inactivation test.RESULTS: ①The water contact angle of the vascular graft surface was greatly decreased after modifying. ATR-FTIR revealed the disappearance of diazonium groups at 2 172 cm-1 and 2 224 cm-1. Vascular prosthesis after modifying had prolonged APTT and PT, low percent hemolysis and low amount of platelet adhesion. Modified vascular prosthesis had inhibitory effect on thrombosin activity and good coating stability.CONCLUSION: Converage of PVA/PA/Hep has good antithrombotic function and low percent hemolysis, resulting in improving hemocompatibility of vascular prosthesis.
