CAJ | 학술논문

本研究测定了昆虫纲半翅几姬缘蝽科Rhopalidae绿环缘蝽Stictopleurussubviridis Hsiao的完整线粒体基凶组.为异翅亚目蝽次目Penmtomomorha中的首例研究.该线粒体基因组为全长15 139 bp的双链环状DNA分子,含有后生动物线粒体基凶组中典型的37个基因,AT含量为75.7%.各基因的排列方式与六足动物假想祖先相同.该线粒体基因组结构紧凑,有基因重叠现象.在tRNA-Ⅱe与tRNA-GLn问发现一个罕见的79bp的非编码序列.tRNA-Ser的二级结构中DHU臂缺失,无法折叠成典型的三叶草结构.CO2和CO3终止密码子为T,其下游为同链编码的tRNA基因.基因组AT含量为75.7%,密码子使用频率也反映出AT偏好.两条链上蛋白质编码基凶的CG-skew趋势相反,密码子使用频率也反映出两链不同的CG偏好.蛋白编码序列并不总是使用与tRNA的反密码子相对应的密码子,密码子的使用并不随机.使用频率最高密码子为NNA,使用频率最低的密码子为NNG.与臭虫次目Cimicomomorpha中的锥猎蝽Triaatoma dimidiata线粒体基因组比较分析表明,ATP8为进化最快的蛋白质,CO1最慢,但tRNA和rRNA基因的进化速率比蛋白质基因低.
본연구측정료곤충강반시궤희연춘과Rhopalidae록배연춘Stictopleurussubviridis Hsiao적완정선립체기흉조.위이시아목춘차목Penmtomomorha중적수례연구.해선립체기인조위전장15 139 bp적쌍련배상DNA분자,함유후생동물선립체기흉조중전형적37개기인,AT함량위75.7%.각기인적배렬방식여륙족동물가상조선상동.해선립체기인조결구긴주,유기인중첩현상.재tRNA-Ⅱe여tRNA-GLn문발현일개한견적79bp적비편마서렬.tRNA-Ser적이급결구중DHU비결실,무법절첩성전형적삼협초결구.CO2화CO3종지밀마자위T,기하유위동련편마적tRNA기인.기인조AT함량위75.7%,밀마자사용빈솔야반영출AT편호.량조련상단백질편마기흉적CG-skew추세상반,밀마자사용빈솔야반영출량련불동적CG편호.단백편마서렬병불총시사용여tRNA적반밀마자상대응적밀마자,밀마자적사용병불수궤.사용빈솔최고밀마자위NNA,사용빈솔최저적밀마자위NNG.여취충차목Cimicomomorpha중적추작춘Triaatoma dimidiata선립체기인조비교분석표명,ATP8위진화최쾌적단백질,CO1최만,단tRNA화rRNA기인적진화속솔비단백질기인저.
The first complete mitochondrial genome of pentatomomorphan bug,Stictopleurus Hsiao,1977(Insecta, Hemiptera-Heteroptera.Rhopalidae)was sequenced in this study.Thc genome was a circular molecule of 15319 bp containing the typical 37 genes that arranged in the same order as that of the putative ancestor of hexapods.Sequences overlaps were observ ed between several neighbor genes,which made the genome relatively compact.An unusual non-coding spacer was found between tRNA-Ⅱe and tRNA-Gin.The tRNA-Ser(GCT)could not be folded into typical secondary structure because its DHU arm was replaced with a simple loop.CO2 and CO3 were terminated with a single T adjacent to a downstream tRNA gene in the same strand.The genome was AT-biased with a total A+T content of75.7%which w-as also demonstrated by the codon usage.Protein genes of two strands presented opposite CG-skew trends which was also reflected by dlc codon usage.For most of the amino acids, the protein coding seqHences did not prefer tO use the cognate codons of corresponding tRNAs and the codon usage of the protein genes was not random.The most frequently used codons were NNA and the most infrequently were NNG.Compared with another complete mitochondrial genome of Heteroptera.Trialoma,ATP8 was the fastest evolving protein and Col was the slowest, while tRNAs and rRNAs were slower than all the proteins.