CAJ | 학술논문

用限制性片段长度多态性(Restriction fragment length polymorphism,RFLP)方法研究了在农业废物堆肥一次发酵和二次发酵期间添加黄孢原毛平革菌(P.chrysosporium)对微生物多样性的影响.结果表明,在不同发酵期接种P.chrysosporium对堆肥进程的影响不同.3种典型的限制性内切酶Alu Ⅰ、Hae Ⅲ和Taq Ⅰ在分析堆肥细菌微生物多样性的灵敏性上,Hae Ⅲ效果最好,Alu Ⅰ次之,Taq Ⅰ则并不很适用于分析堆肥细菌微生物多样性.接种黄孢对堆肥细菌群落的影响非常显著且迅速,二次接种可起到巩固一次接种效果的作用.
용한제성편단장도다태성(Restriction fragment length polymorphism,RFLP)방법연구료재농업폐물퇴비일차발효화이차발효기간첨가황포원모평혁균(P.chrysosporium)대미생물다양성적영향.결과표명,재불동발효기접충P.chrysosporium대퇴비진정적영향불동.3충전형적한제성내절매Alu Ⅰ、Hae Ⅲ화Taq Ⅰ재분석퇴비세균미생물다양성적령민성상,Hae Ⅲ효과최호,Alu Ⅰ차지,Taq Ⅰ칙병불흔괄용우분석퇴비세균미생물다양성.접충황포대퇴비세균군락적영향비상현저차신속,이차접충가기도공고일차접충효과적작용.
The lignocellulolytic microorganism P. chrysosporium was inoculated during different phases of agricultural waste composting and the effect on the diversity of microbial community was observed. The results of the present investigation revealed that inoculation during dif-ferent phases had different effects on these parameters. Three kinds of restriction enzymes. Alu Ⅰ , Hae Ⅲ and Taq Ⅰ were used to analyse the change of microbial community diversity. The results showed that restriction enzyme Hae Ⅲ was the most sensitive to the change of microbial community, restriction enzyme Alu Ⅰ was the second, and restriction enzyme Taq Ⅰ was the last. Restriction enzyme Taq Ⅰ was not much suitable for analyzing composting samples. P. chrysosporium inoculated to composting system acted on certain microorganisms selectively and made them be the dominant species in order to promote the degradation of compost materials. The effect of inoculation on bacteria community was significant and rapid, the inoculation in the second fermentation was able to advance the effects of the inoculation in the first fermenta-tion.