国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2009年
7期
544-547
,共4页
李波波%李道堂%赵东波%王兴武
李波波%李道堂%趙東波%王興武
리파파%리도당%조동파%왕흥무
食管肿瘤%DKK-1%肿瘤标记物%诊断学
食管腫瘤%DKK-1%腫瘤標記物%診斷學
식관종류%DKK-1%종류표기물%진단학
Esophageal neoplasms%DKK-1%Tumor marker%Diagnosis
目的 检测食管癌患者血清中DKK-1(dickkopf-1)的表达水平,探讨其在食管癌辅助诊断中的价值.方法 应用酶联免疫吸附法(ELISA)检测80例食管鳞癌患者和35例健康对照组血清中DKK-1的浓度,计算DKK-1诊断食管鳞癌的敏感性、特异性、阳性预测值和阴性预测值,并与细胞角蛋白19片段(CYFRA21-1)和癌胚抗原(CEA)的检测做比较,探讨其在食管癌辅助诊断中的价值.结果 ①食管鳞癌患者血清中DICK-1浓度为90.44±57.41 ng/ml,而正常对照组为11.29±9.45 ng/ml,两者差异有统计学意义(P<0.01).②以正常人血清DKK-1浓度95%可信区间上限(14.54 ng/ml)为界值,则食管鳞癌患者血清中DICK-1的敏感性为66.25%(53/80);正常人血清中的DICK-1阳性率仅为17.14%(6/35),两者差异有统计学意义(P<0.01).③食管鳞癌患者血清中DKK-1的阳性率与肿瘤大小、分化程度无关(P>0.05),而与肿瘤分期和淋巴结转移密切相关(P<0.05).④3种肿瘤标记物检测比较分析显示,在诊断食管癌时,DKK-1的敏感性为66.25%,与CEA(39.44%)和CYFRA21-1(25.35%)间差异有统计学意义(P<0.05).结论 DKK-1在食管癌的发生、生长和转移过程中起着极其重要的作用,有可能成为一种新的肿瘤标记物用于食管癌的辅助诊断.
目的 檢測食管癌患者血清中DKK-1(dickkopf-1)的錶達水平,探討其在食管癌輔助診斷中的價值.方法 應用酶聯免疫吸附法(ELISA)檢測80例食管鱗癌患者和35例健康對照組血清中DKK-1的濃度,計算DKK-1診斷食管鱗癌的敏感性、特異性、暘性預測值和陰性預測值,併與細胞角蛋白19片段(CYFRA21-1)和癌胚抗原(CEA)的檢測做比較,探討其在食管癌輔助診斷中的價值.結果 ①食管鱗癌患者血清中DICK-1濃度為90.44±57.41 ng/ml,而正常對照組為11.29±9.45 ng/ml,兩者差異有統計學意義(P<0.01).②以正常人血清DKK-1濃度95%可信區間上限(14.54 ng/ml)為界值,則食管鱗癌患者血清中DICK-1的敏感性為66.25%(53/80);正常人血清中的DICK-1暘性率僅為17.14%(6/35),兩者差異有統計學意義(P<0.01).③食管鱗癌患者血清中DKK-1的暘性率與腫瘤大小、分化程度無關(P>0.05),而與腫瘤分期和淋巴結轉移密切相關(P<0.05).④3種腫瘤標記物檢測比較分析顯示,在診斷食管癌時,DKK-1的敏感性為66.25%,與CEA(39.44%)和CYFRA21-1(25.35%)間差異有統計學意義(P<0.05).結論 DKK-1在食管癌的髮生、生長和轉移過程中起著極其重要的作用,有可能成為一種新的腫瘤標記物用于食管癌的輔助診斷.
목적 검측식관암환자혈청중DKK-1(dickkopf-1)적표체수평,탐토기재식관암보조진단중적개치.방법 응용매련면역흡부법(ELISA)검측80례식관린암환자화35례건강대조조혈청중DKK-1적농도,계산DKK-1진단식관린암적민감성、특이성、양성예측치화음성예측치,병여세포각단백19편단(CYFRA21-1)화암배항원(CEA)적검측주비교,탐토기재식관암보조진단중적개치.결과 ①식관린암환자혈청중DICK-1농도위90.44±57.41 ng/ml,이정상대조조위11.29±9.45 ng/ml,량자차이유통계학의의(P<0.01).②이정상인혈청DKK-1농도95%가신구간상한(14.54 ng/ml)위계치,칙식관린암환자혈청중DICK-1적민감성위66.25%(53/80);정상인혈청중적DICK-1양성솔부위17.14%(6/35),량자차이유통계학의의(P<0.01).③식관린암환자혈청중DKK-1적양성솔여종류대소、분화정도무관(P>0.05),이여종류분기화림파결전이밀절상관(P<0.05).④3충종류표기물검측비교분석현시,재진단식관암시,DKK-1적민감성위66.25%,여CEA(39.44%)화CYFRA21-1(25.35%)간차이유통계학의의(P<0.05).결론 DKK-1재식관암적발생、생장화전이과정중기착겁기중요적작용,유가능성위일충신적종류표기물용우식관암적보조진단.
Objective To explore the expression of DKK-1 and its clinical value in patients with e-sophageal cancer. Methods The levels of serum DKK-1 were measured by sandwich enzyme linked immu-nosorbent assay(ELISA) in 80 patients with esophageal squamous cell cancer and in 35 healthy subjects. The sensitivities of serum DKK-1, CEA and CYFRA21-1 in the patients with esophageal squamous cell cancer were calculated and analyzed to compare their values in diagnosis of esophageal cancer. Results The serum DKK- 1 levels in patients with esophageal squamous cell cancer were significantly higher than those of healthy subjects(P<0.05). According to the highest value of medicine reference of DKK- 1 (14. 54 ng/ml), its sensitivity and specificity were 66. 25% and 82. 86% respectively; There was a significant difference between the sensitivity of DKK-1 in patients with esophageal squamons cell cancer(66. 25%) and that in healthy subjects(17.14%) (P<0.05). The sensitivitis of serum DKK- 1 in patients with esophageal squamous cell cancer were associated with tumor size, TNM stage and lymph node metastasis(P <0.05),but not associated with gender,age and differentiation grade(P>0.05). And the sensitivity of DKK-1 (66. 25%) was significantly higher than that of CEA, CYFRA21-1 (39. 44% ,25. 35%, P <0. 01). Conclusion DKK-1 played a very important role in the growth and metastasis of esophageal cancer and may be a new tumor marker or an important index in the diagno-sis of esophageal cancer.
